COBRE on Membrane Protein Production and Characterization

COBRE 膜蛋白生产和表征

• 批准号: 8528632
• 负责人: ABRAHAM M LENHOFF
• 金额: $ 107.95万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-10 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8528632
• 关键词: Address; Adopted; Advisory Committees; Area; Biological Process; Biology; Biomedical Research; Cells; Centers of Research Excellence; Communication; Communities; Complement; Complex; Core Facility; Core Protein; Coupled; Crystallization; Data; Delaware; Detergents; Development; Disease; Faculty; FarGo; Funding; Genomics; Health; Human Genome; Institutional National Research Service Award; Laboratories; Lead; Marketing; Membrane; Membrane Proteins; Methods; Modeling; Molecular; Phase; Pilot Projects; Production; Protein Structure Initiative; Proteins; Research; Research Infrastructure; Research Personnel; Research Support; Role; Series; Structure; Therapeutic; Training Programs; United States National Institutes of Health; Universities; Work; biological systems; cell growth; drug development; improved; infrastructure development; instrumentation; interest; nutrition; outreach; overexpression; pre-doctoral; programs; protein expression; protein function; protein structure; solid state nuclear magnetic resonance; structural biology; symposium

We propose to continue the COBRE on Membrane Protein Production and Characterization at the University of Delaware as a Phase III COBRE infrastructure center. The critical role of membrane proteins in biology is clearly reflected in the fact that they represent >30% of the human genome and are the targets of >50% of all therapeutics on the market today. Nevertheless, significant challenges remain in producing and characterizing them, especially within the realm of structural biology. Consequently the objectives of research in the COBRE will be to express, solubilize, purify and crystallize membrane proteins; to determine their structures; and to characterize their functions at the molecular level and in larger biological systems in both health and disease. These objectives are congruent with those of the Structural Biology component of the NIH Roadmap and the Protein Structure Initiative in addressing an area of critical importance for biomedical research. The infrastructure center will complement research in the laboratories of an interdisciplinary group of more than a dozen PIs, whose work is independently supported. To this end the center will include: research and instrumentation cores (Protein Expression and Biophysical Characterization; Structural Biology; and a group of Core Facilities co-funded with, among others, the Delaware INBRE); continuation of the current pilot research subproject program; a predoctoral training program modeled on the NIH T32 mechanism; and continuation of the existing annual symposium series. The administration of the center will be guided by an internal Steering Committee and will continue to benefit from the input of an External Advisory Committee, including for review of pilot project proposals. The research within the COBRE coupled with the capabilities provided by the infrastructure center will continue to give the University of Delaware distinctive strength in an area of research critical to biology and biomedicine.

我们建议在膜蛋白产生和表征上继续 特拉华大学作为第三阶段的基础设施中心。膜蛋白的关键作用 在生物学中，显然反映出它们代表了人类基因组的30％，并且是目标 当今市场上所有治疗剂的50％> 50％。然而，生产仍然存在重大挑战 并描述它们，尤其是在结构生物学领域。因此 在毛病中的研究将是表达，溶解，纯化和结晶膜蛋白。确定 他们的结构；并在分子水平和较大的生物系统中表征其功能 健康和疾病。这些目标与结构生物学成分的目标是一致的 NIH路线图和蛋白质结构计划，以解决至关重要的领域 生物医学研究。 基础设施中心将补充更多跨学科小组实验室的研究 比十几个PI，其工作得到了独立支持。为此，该中心将包括：研究和 仪器核心（蛋白质表达和生物物理表征；结构生物学；一组 核心设施与特拉华州的近亲共同资助）；当前飞行员的延续 研究副本计划；以NIH T32机制为模型的培训前培训计划；和 现有年度研讨会系列的延续。该中心的管理将由 内部指导委员会，并将继续从外部咨询委员会的意见中受益， 包括用于审查试点项目建议。鞋垫中的研究与功能相结合 由基础设施中心提供的 对生物学和生物医学至关重要的研究领域。