The Interplay Between the Circadian Clock and Plant Immune Mechanisms

昼夜节律钟与植物免疫机制之间的相互作用

• 批准号: 8546422
• 负责人: Xinnian Dong
• 金额: $ 28.04万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-17 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8546422
• 关键词: Affect; Agriculture; Animals; Apoptosis; Arabidopsis; Autoimmune Diseases; Behavior; Binding; Biological Process; Biological Response Modifiers; Brain; Brassicaceae; Cell Survival; Cells; Circadian Rhythms; Cluster Analysis; Complex; Data; Defense Mechanisms; Detection; Disease; Disease Resistance; Dissection; Employee Strikes; Environment; Equilibrium; Eukaryota; Famines; Gene Expression; Genes; Genetic; Genetic Epistasis; Genetic Transcription; Health; Human; Immune; Immune response; Immunity; Infection; Interleukin-1 Receptors; Jet Lag Syndrome; Knock-out; Leucine-Rich Repeat; Life; Link; Mediating; Metabolism; Molecular; Molecular Genetics; Molecular Profiling; Movement; Mutate; Natural Immunity; Nuclear Translocation; Nucleotides; Obesity; Oomycetes; Organ; Organism; Oxidation-Reduction; Pattern; Peripheral; Pesticides; Physiologic pulse; Phytophthora; Plant Leaves; Plants; Population; Potato; Predisposition; Production; Proteins; Regulation; Relative (related person); Reporter; Reporting; Reproduction spores; Resistance; Resources; Role; Salicylic Acids; Signal Transduction; Site; System; Testing; Time; Tissues; Whole Organism; base; circadian pacemaker; cofactor; design; fitness; functional group; mathematical model; mutant; overexpression; pathogen; promoter; response; vpr Genes; Affect; Agriculture; Animals; Apoptosis; Arabidopsis; Autoimmune Diseases; Behavior; Binding; Biological Process; Biological Response Modifiers; Brain; Brassicaceae; Cell Survival; Cells; Circadian Rhythms; Cluster Analysis; Complex; Data; Defense Mechanisms; Detection; Disease; Disease Resistance; Dissection; Employee Strikes; Environment; Equilibrium; Eukaryota; Famines; Gene Expression; Genes; Genetic; Genetic Epistasis; Genetic Transcription; Health; Human; Immune; Immune response; Immunity; Infection; Interleukin-1 Receptors; Jet Lag Syndrome; Knock-out; Leucine-Rich Repeat; Life; Link; Mediating; Metabolism; Molecular; Molecular Genetics; Molecular Profiling; Movement; Mutate; Natural Immunity; Nuclear Translocation; Nucleotides; Obesity; Oomycetes; Organ; Organism; Oxidation-Reduction; Pattern; Peripheral; Pesticides; Physiologic pulse; Phytophthora; Plant Leaves; Plants; Population; Potato; Predisposition; Production; Proteins; Regulation; Relative (related person); Reporter; Reporting; Reproduction spores; Resistance; Resources; Role; Salicylic Acids; Signal Transduction; Site; System; Testing; Time; Tissues; Whole Organism; base; circadian pacemaker; cofactor; design; fitness; functional group; mathematical model; mutant; overexpression; pathogen; promoter; response; vpr Genes

DESCRIPTION (provided by applicant): The circadian clock has profound impacts on living organisms on earth. It maximizes the fitness of an organism by enabling it to coordinate metabolism and behavior with its environment. However, studies that unequivocally demonstrate the fitness benefits are scarce and the underlying molecular mechanisms are poorly known. The surprising discovery that the Arabidopsis clock component, CCA1, is a key regulator of RPP4-mediated resistance against the oomycete pathogen Hyaloperonospora arabidopsidis (Hpa) leads to this first in depth study of the interplay between the circadian clock and plant immunity mechanisms. Aim 1 of this study will focus on the dissection of the regulatory circuitry between CCA1 and the resistance protein RPP4 using molecular genetics and systems approaches. A mathematical model will be made to explain how RPP4 perturbs CCA1-mediated rhythmic expression of defense genes resulting in programmed cell death of the infected cell and resistance to Hpa. Aim 2 will investigate the role of the circadian clock in systemic acquired resistance to test the hypothesis that the clock is reset during this immune response to redirect the resource to defense and promote cell survival. There are striking parallels between plant defense mechanisms and animal innate immunity. The influence of the circadian clock on animal immune responses has also been reported. However, in depth studies are challenging because of the inherent complexities of animal circadian clocks (central and peripheral) and of the microflora. Therefore, the proposed Arabidopsis-Hyaloperospora study will have a great advantage in revealing the design principles underline the interplay between the circadian clock and immunity besides providing detailed molecular mechanisms. The system perspectives on plants immune mechanisms will help us design better strategies to control crop disease and reduced the use of pesticides, which are hazardous to the environment and human health. Plant health has always had a significant impact on human health as demonstrated by the infamous Irish potato famine in 1840s, which was caused by Phytophthora infestans, a close relative of Hpa used in this study. With the world population exploding, developing better and safer agricultural practices is not a choice, but a necessity.

描述（由申请人提供）：昼夜节律对地球上的生物有深远的影响。它通过使其能够与环境协调代谢和行为来最大化生物体的适应性。然而，明确证明适应性益处的研究很少，而潜在的分子机制是鲜为人知的。令人惊讶的发现，拟南芥的时钟成分CCA1是RPP4介导的抗性对Oomycete病原体透明质球型拟南芥（HPA）的关键调节剂，它导致了昼夜节律之间的相互作用深度研究的第一个第一次研究。 和植物免疫机制。这项研究的目标1将使用分子遗传学和系统方法来解剖CCA1和抗性蛋白RPP4之间的调节电路。将制定一个数学模型，以解释RPP4如何渗透CCA1介导的防御基因的节奏表达，从而导致受感染细胞的程序性细胞死亡和对HPA的抗性。 AIM 2将研究昼夜节律时钟在系统性获得的耐药性中的作用，以测试在这种免疫反应中重置时钟以重定向资源以防御和促进细胞存活的假设。植物防御机制和动物先天免疫之间存在惊人的相似之处。还报道了昼夜节律对动物免疫反应的影响。然而，由于动物昼夜节律钟（中心和周围）和菌群的固有复杂性，因此深入研究很具有挑战性。因此，拟议的拟南芥二肌膜研究将在揭示设计原理强调昼夜节律与免疫之间的相互作用方面具有很大的优势，除了提供详细的分子机制。系统对植物免疫机制的看法将有助于我们设计更好的策略来控制农作物疾病，并减少对环境和人类健康危害的农药的使用。植物健康一直对人类健康产生重大影响，如1840年代臭名昭著的爱尔兰马铃薯饥荒所证明的那样，该饥荒是由疫霉菌（Phytophthora Infestans）造成的，这是本研究中使用的HPA的近亲。随着世界人口的爆发，发展更好，更安全的农业实践不是选择，而是必要的。