Bioengineering Polymers for Parsing Cell Responses

用于解析细胞反应的生物工程聚合物

基本信息

批准号：
8270346
负责人：
LINDA G GRIFFITH
金额：
$ 34.27万
依托单位：
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-09-01 至 2014-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8270346
关键词：
Acute Address Adhesions Adhesives Affect Apoptosis Area Behavior Biocompatible Materials Biological Biological Models Biomedical Engineering Blood Bone Regeneration Cell Communication Cell Proliferation Cell Surface Receptors Cell Survival Cell surface Cells Cessation of life Chronic Communication Computer software Cues Cytokine Signaling Data Defect Dependence EGF gene Electronics Engineering Environment Epidermal Growth Factor Receptor Equilibrium Fibronectins Funding Goals Grant Growth Growth Factor Growth Factor Receptors Healed Human Immigration Inflammatory Institutes Integrins Internet Laboratories Learning Ligands Location Mails Massachusetts Mediating Mesenchymal Stem Cells Modality Molecular Natural regeneration Nature Outcome Paper Pathway interactions Pattern Phenotype Play Polymers Production Publications Receptor Activation Receptor Signaling Relative (related person)Research Personnel Role Science Signal Pathway Signal Transduction Site Source Stimulus Surface System Techniques Technology Telephone Testing Tissue Engineering Tissues Undifferentiated Universities Visit Work adhesion receptor autocrine base cell behavior cell type clinical application clinically relevant data sharing extracellular graduate student healing in vivo insight meetings migration novel prevent receptor receptor internalization response scaffold skin regeneration stem cell differentiation stem cell population tissue regeneration wound

项目摘要

DESCRIPTION (provided by applicant): Work will be conducted jointly at the Massachusetts Institute of Technology and at the University of Pittsburgh. Specific tasks which will be performed at each location under the direction of Wells and Griffith are summarized below. Work to be performed at University of Pittsburgh (A. Wells Laboratory): 1. Activation of select signaling pathway downstream from EGFR will be quantified in the undifferentiated MSC (Aim 1). 2. Activation of select signaling pathway downstream from EGFR will be quantified in the pre-differentiated MSC (Aim 2). 3. Production of autocrine ligands will be quantified with the MSC on various surfaces (Aim 2). 4. Differentiation of MSC while on the various surfaces into at least 3 lineages (Aim 3). Work to be performed at MIT (L. Griffith and P. Hammond Laboratory): 1. Synthesis of t-EGF substrates (Aims 1-3). Similar substrates have been successfully mailed these substrates to Pittsburgh and other labs and maintained their activity. 2. Analysis of apoptosis (Aim 1) and proliferation (Aim 2) via FACS 3. Synthesis of substrates for specialized migration/differentiation studies (Aim 3) These tasks will be coordinated by frequent telephone and electronic communications, facilitated by compatible computer software. This will promote data sharing and interpretation by all involved. All major planning will be done at semi-annual visits by the Investigators; as has been accomplished since 1994. The most recent meeting occurred in Cambridge in January 2007. This frequent visiting is further facilitated by serving on the thesis committees of each other's graduate students. Lastly, trainees will be exchanged for extended periods to learn complementary techniques and approaches; this `sharing' of trainees has been used to great advantage. Narrative Bioengineered Polymers for Parsing Cell Responses The major goal of this project is to validate an observation obtained in a model system regarding the dependence of cell interactions (adhesion, migration, proliferation) with RGD and EGFR ligands on the spatial arrangement of the ligands and extend the results to a clinically-relevant system.

描述（由申请人提供）：工作将在马萨诸塞州理工学院和匹兹堡大学共同开展。下面总结了在井和格里菲斯的指示下在每个位置执行的特定任务。在匹兹堡大学（A. Wells实验室）进行的工作： 1。将在未分化的MSC中量化从EGFR下游的选择信号通路的激活（AIM 1）。 2。将在预定的MSC中量化EGFR下游的选择信号通路的激活（AIM 2）。 3。在各种表面上使用MSC量化自分泌配体的产生（AIM 2）。 4。在各个表面上将MSC分化为至少3个谱系（AIM 3）。在麻省理工学院（L. Griffith和P. Hammond实验室）进行的工作： 1。T-EGF底物的合成（目标1-3）。类似的基材已成功地邮寄给匹兹堡和其他实验室，并维持其活动。 2。凋亡分析（AIM 1）和FACS的增殖（AIM 2） 3。用于专门迁移/分化研究的底物的合成（AIM 3）这些任务将通过频繁的电话和电子通信协调，并通过兼容的计算机软件促进。这将促进所有参与者的数据共享和解释。所有重大计划将在调查人员的半年度访问中进行；自1994年以来就已经完成了。最近的会议发生在2007年1月在剑桥。通过在彼此的研究生论文委员会任职，这一频繁的访问进一步促进了这一频繁的探访。最后，学员将长时间交换，以学习互补的技术和方法。这种“分享”学员已被用来极大的优势。叙述用于解析细胞反应的生物工程聚合物该项目的主要目的是验证在模型系统中获得的观察结果与RGD和EGFR配体在空间上的细胞相互作用（粘附，迁移，增殖）的依赖性配体的排列并将结果扩展到与临床相关的系统。