HIGH-RESOLUTION EX VIVO IMAGING OF AN ALZHEIMER'S MOUSE MODEL

阿尔茨海默病小鼠模型的高分辨率离体成像

• 批准号: 8171559
• 负责人: JOANNA L JANKOWSKY
• 金额: $ 1.09万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171559
• 关键词: Alzheimer' s Disease; Amyloid deposition; Amyloidosis; Animals; Area; Atlases; Brain; Collaborations; Complement; Computer Retrieval of Information on Scientific Projects Database; Data; Data Analyses; Disease; Funding; Gene Expression; Goals; Grant; Image; Institution; Magnetic Resonance Imaging; Molecular Profiling; Mus; Neurodegenerative Disorders; Research; Research Personnel; Resolution; Resources; Source; Testing; Tetanus Helper Peptide; Time; United States National Institutes of Health; Work; mouse model

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Using MR 9T magnet for this study. The MouseBIRN collaborative group seeks testbed cases to generate a searchable, online atlas that combines gene expression and 3D MRI images of mouse models for neurodegenerative disease. We would like to develop the new tet-off APP mouse model of Alzheimer's disease in this context. Work is already under way to generate gene expression profiles of the tet-off APP mice at multiple points in the progression of amyloidosis. The imaging proposed in collaboration with CIVM would provide comprehensive, high-resolution, 3D morphological images to complement the gene expression analyses. We will focus imaging efforts on two of the five time points assessed for gene expression, one before and one after the onset of amyloid deposition. The goal is to develop an average rendering from 5 Alzheimer's (tet-off APP) and 5 control (tTA) animals at each time point that can be used not only as a test of the MouseBIRN automated data analysis pipeline but also provide valuable information about volumetric and/or structural changes in specific brain areas as the disease progresses. Data from this study will be made publicly available through the MouseBIRN network.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 本研究使用 MR 9T 磁铁。 MouseBIRN 协作小组寻找测试床案例来生成可搜索的在线图集，该图集结合了神经退行性疾病小鼠模型的基因表达和 3D MRI 图像。 在此背景下，我们希望开发新的 tet-off APP 阿尔茨海默病小鼠模型。 目前已经在开展工作，以生成 tet-off APP 小鼠在淀粉样变性进展过程中多个点的基因表达谱。 与 CIVM 合作提出的成像将提供全面、高分辨率的 3D 形态学图像，以补充基因表达分析。 我们将把成像工作重点放在评估基因表达的五个时间点中的两个上，一个在淀粉样蛋白沉积开始之前，一个在淀粉样蛋白沉积开始之后。 目标是开发 5 只阿尔茨海默氏症（tet-off APP）和 5 只对照（tTA）动物在每个时间点的平均渲染，不仅可以用作 MouseBIRN 自动化数据分析管道的测试，还可以提供有关以下方面的有价值的信息：随着疾病的进展，特定大脑区域的体积和/或结构发生变化。这项研究的数据将通过 MouseBIRN 网络公开提供。