CRYSTAL STRUCTURE OF YEAST MITOCHONDRIA TRANSLOCON MEMBER TIM50

酵母线粒体转运蛋白 TIM50 的晶体结构

• 批准号: 8171510
• 负责人: BINGDONG SHA
• 金额: $ 2.5万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171510
• 关键词: Biogenesis; Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; Cytosol; Funding; Grant; Institution; Membrane; Membrane Proteins; Mitochondria; N-terminal; Play; Protein translocation; Research; Research Personnel; Resources; Role; Source; Structure; United States National Institutes of Health; Yeasts; member; protein transport; receptor; translocase

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Protein translocations across mitochondria membranes play critical roles in mitochondria biogenesis. The protein transports from the cell cytosol to the mitochondria matrix are carried out by the translocase of the outer membrane (TOM) complex and the translocase of the inner membrane (TIM) complex. In the TIM23 translocon, Tim50 functions as a receptor to guide the precursor with the N-terminal mitochondrion targeting sequence to the inner membrane protein channel for translocation. Recently we have crystallized yeast Tim50. The crystallographic studies on yeast TIM23 translocon will uncover the basic mechanisms how the translocons facilitate the protein translocations across the mitochondria outer and inner membranes.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 跨线粒体膜的蛋白质易位在线粒体生物合成中发挥着关键作用。蛋白质从细胞质到线粒体基质的转运是通过外膜转位酶（TOM）复合物和内膜转位酶（TIM）复合物进行的。 在TIM23易位子中，Tim50作为受体引导带有N端线粒体靶向序列的前体进入内膜蛋白通道进行易位。最近我们有结晶酵母Tim50。对酵母 TIM23 易位子的晶体学研究将揭示易位子如何促进蛋白质跨线粒体外膜和内膜易位的基本机制。