CHARACTERIZATION OF KEY REGULATORY PROTEINS IN T GONDII

弓形虫中关键调节蛋白的表征

• 批准号: 8171387
• 负责人: Rima L McLeod
• 金额: $ 0.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171387
• 关键词: Animals; Be++ element; Beryllium; Binding; Computer Retrieval of Information on Scientific Projects Database; DNA Binding; EMSA; Elements; Family; Funding; Gel; Genes; Grant; Growth; Institution; Linker-Scanning Mutagenesis; Nuclear Protein; Nuclear Proteins; Parasites; Plants; Protein Binding; Proteins; Regulatory Pathway; Research; Research Personnel; Resources; Ribosomal Proteins; Source; Structural Models; United States National Institutes of Health; genetic regulatory protein; histone acetyltransferase; member; promoter; transcription factor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Analysis of promoter cis elements of ribosomal protein (RP) genes identified two common ribosomal protein promoter motifs called TRP1 and TRP2. Nuclear proteins that bound to these TRP elements were characterized using EMSA, and the motif responsible for protein binding to TRP2 within the promoter encoding small ribosomal protein 13 (rps13) was identified using linker scan mutagenesis. This motif, TGCATGA, is the same as the motif known to bind an Apetela-2 (AP-2) apicomplexan transcription factor, a member of a plant-like family of transcription factors present in apicomplexan parasites but not in animals. Structural modeling of AP-2s identified a critical motif for DNA binding. TRP element interactions with histone acetyltransferases, MYST-A and MYST-B were demonstrated by gel shift using ∈?MYST-A/B in EMSAs and ChIP studies. Thus, proteins likely to participate in key regulatory pathways determining tachyzoite growth and growth arrest were identified.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 对核糖体蛋白 (RP) 基因启动子顺式元件的分析确定了两个常见的核糖体蛋白启动子基序，称为 TRP1 和 TRP2。 使用 EMSA 对与这些 TRP 元件结合的核蛋白进行了表征，并使用接头扫描诱变鉴定了编码小核糖体蛋白 13 (rps13) 的启动子内负责与 TRP2 蛋白结合的基序。 该基序 TGCATGA 与已知结合 Apetela-2 (AP-2) 顶复门转录因子的基序相同，Apetela-2 (AP-2) 顶复门转录因子是类植物转录因子家族的成员，存在于顶复门寄生虫中，但不存在于动物中。 AP-2 的结构建模确定了 DNA 结合的关键基序。 TRP 元件与组蛋白乙酰转移酶、MYST-A 和 MYST-B 的相互作用通过在 EMSAs 和 ChIP 研究中使用 εMYST-A/B 进行凝胶位移来证明。 因此，鉴定了可能参与决定速殖子生长和生长停滞的关键调节途径的蛋白质。