STRUCTURAL BIOLOGY OF SELECTED TARGETS FROM MYCOBACTERIUM TUBERCULOSIS

结核分枝杆菌选定靶标的结构生物学

• 批准号: 8170300
• 负责人: Katherine S Bateman
• 金额: $ 0.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170300
• 关键词: Amino Acids; Binding; Computer Retrieval of Information on Scientific Projects Database; Data Collection; Drug Metabolic Detoxication; Funding; Gene Targeting; Goals; Grant; Institution; Mycobacterium tuberculosis; Pathway interactions; Phase; Proteins; Research; Research Personnel; Resolution; Resources; Screening procedure; Source; Structure; United States National Institutes of Health; Work; design; expression cloning; inhibitor/antagonist; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have selected more than 400 target genes from Mycobacterium tuberculosis for cloning, expression and crystal structure determination. The targets are either gene products with an unknown function or are enzymatic activities from several essential pathways involved in detoxification or amino acid synthesis. Over 25 crystal structures have been determined in our lab to date and a number of targets have been cloned, expressed and crystallized. Structural work is continuing on the previously determined crystal structures with the goal of elucidating the detailed enzymatic mechanism for the design of effective inhibitors. The project requires the screening of typically large numbers of crystals, MAD or SAD phasing and high-resolution data collection to visualize the details of substrate or inhibitor binding.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们从结核分枝杆菌中筛选出400多个靶基因进行克隆、表达和晶体结构测定。目标要么是功能未知的基因产物，要么是参与解毒或氨基酸合成的几个重要途径的酶活性。迄今为止，我们的实验室已确定了超过 25 种晶体结构，并且许多靶标已被克隆、表达和结晶。先前确定的晶体结构的结构工作仍在继续，目的是阐明设计有效抑制剂的详细酶促机制。该项目需要筛选大量晶体、MAD 或 SAD 定相以及高分辨率数据收集，以可视化底物或抑制剂结合的细节。