COBRE: OK MED RES FOUND: CORE II: INTRAVITAL MICROSCOPE CORE FACILITY

COBRE：OK MED RES Found：CORE II：活体显微镜核心设施

• 批准号: 8168455
• 负责人: Lijun Xia
• 金额: $ 8.97万
• 依托单位: OKLAHOMA MEDICAL RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-08 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168455
• 关键词: Adhesives; Area; Atherosclerosis; Cells; Color; Complex; Computer Retrieval of Information on Scientific Projects Database; Computers; Core Facility; Funding; Grant; Hematopoiesis; Homing; Immersion Investigative Technique; Immune; Inflammation; Institution; Leukocytes; Lymphocyte; Microscope; Operative Surgical Procedures; Physiological; Preparation; Research; Research Personnel; Resources; Source; Stem Cell Development; System; United States National Institutes of Health; Vascular Endothelium; Water; data acquisition; intravital microscopy; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The intravital microscopy system represents a sophisticated tool to dissect complex physiological or pathological cell-cell or cell-matrix interactions such as leukocyte recruitment in inflammation and atherosclerosis, lymphocyte homing in immune development, and stem cell homing in hematopoiesis. Our core facility is equipped with an advanced Nikon ECLIPSE E600-FN intravital epifluorescence microscope with water immersion objectives connected to a Dage-MTI DC-330 3CCD color camera, a SVHS video cassette recorder, and a Microvessel Velocity OD-RT Doppler apparatus. The system also has a Nikon SMZ800 stero microscope for surgical preparation, and an advanced Dell computer for data acquisition and analysis. Using the system, we are able to directly quantify adhesive interactions of circulating leukocytes with vascular endothelium in several ways (i.e., rolling velocity, rolling flux, number of adherent leukocytes in a defined area).

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 活体显微镜系统是剖析复杂的生理或病理性细胞-细胞或细胞-基质相互作用的复杂工具，例如炎症和动脉粥样硬化中的白细胞募集、免疫发育中的淋巴细胞归巢以及造血中的干细胞归巢。我们的核心设施配备了先进的 Nikon ECLIPSE E600-FN 活体落射荧光显微镜， 水浸物镜连接到 Dage-MTI DC-330 3CCD 彩色相机、SVHS 录像机和微血管速度 OD-RT 多普勒装置。该系统还配有用于手术准备的尼康 SMZ800 体视显微镜，以及用于数据采集和分析的先进戴尔计算机。使用该系统，我们能够以多种方式直接量化循环白细胞与血管内皮的粘附相互作用（即滚动速度、滚动通量、特定区域中粘附白细胞的数量）。