IDENTIFICATION OF QSHBC-REGULATED GENES IN NONTYPEABLE HAEMOPHILUS INFLUENZAE

不可分型流感嗜血杆菌中 QSHBC 调控基因的鉴定

• 批准号: 8168253
• 负责人: JASON W. JOHNSTON
• 金额: $ 3.73万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168253
• 关键词: Attenuated; Cell Communication; Cells; Chinchilla (genus); Computer Retrieval of Information on Scientific Projects Database; Escherichia coli; Exhibits; Funding; Genes; Grant; Homologous Gene; In Vitro; Institution; Lead; Microbial Biofilms; Modeling; Nontypable Haemophilus influenza; Operon; Otitis Media; Pathogenesis; Periplasmic Proteins; Research; Research Personnel; Resources; Role; Signal Pathway; Signal Transduction; Signal Transduction Pathway; Source; System; United States National Institutes of Health; Virulence; Work; base; mutant; novel; pathogen

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A newly described signaling pathway in Escherichia coli, the QseBC two-component signal transduction (TCST) system, has been proposed to be involved in both interspecies cell-cell communication and host-pathogen interaction. A homolog to the QseBC system has been identified in NTHi. This system, which I have designated QshABC, has a novel operon organization that includes a gene, qshA, that encodes for a small periplasmic protein. A qshBC mutant exhibits severely attenuated biofilm formation and/or persistence in the chinchilla model for otitis media while it is unaffected in its ability to form a biofilm in vitro. Based on these observations, I propose to further characterize the QshABC system in NTHi to assist in the exploration of its role in pathogenesis. Successful completion of the proposed work would be a crucial step in the characterization of this system and will lead to a better understanding of the signal transduction pathways that are required for the virulence of NTHi.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 大肠杆菌中新描述的信号通路，即 QseBC 双组分信号转导 (TCST) 系统，已被认为参与种间细胞间通讯和宿主与病原体相互作用。 NTHi 中已鉴定出 QseBC 系统的同源物。我将这个系统命名为 QshABC，它有一个新颖的操纵子组织，其中包括一个编码小周质蛋白的基因 qshA。 qshBC突变体在中耳炎龙猫模型中表现出严重减弱的生物膜形成和/或持久性，而其在体外形成生物膜的能力不受影响。基于这些观察，我建议进一步表征 NTHi 中的 QshABC 系统，以协助探索其在发病机制中的作用。成功完成拟议的工作将是表征该系统的关键一步，并将导致更好地了解 NTHi 毒力所需的信号转导途径。