EFFECTS OF SPECIFIC SPERMATID-EXPRESSED Y CHROMOSOME GENES ON SPERM FUNCTION

特定精子细胞表达的 Y 染色体基因对精子功能的影响

• 批准号: 8167754
• 负责人: Monika A Ward
• 金额: $ 22.91万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167754
• 关键词: Affect; Assisted Reproductive Technology; Chromosomes; Complement; Computer Retrieval of Information on Scientific Projects Database; Failure; Fertilization; Fertilization in Vitro; Funding; Genes; Genetic; Germ Cells; Goals; Grant; Health; Human; Infertility; Institution; Intracytoplasmic Sperm Injections; Molecular Biology; Pathway interactions; Play; Research; Research Personnel; Resources; Role; Source; Spermatids; Spermatogenesis; Testing; Testis; United States National Institutes of Health; Work; Y Chromosome; arm; combat; male; men; mouse model; sex determination; sperm function; sry Genes

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Y chromosome was once thought to be responsible only for turning on sex determination towards the male pathway via the testis determinant gene Sry. However, subsequent studies revealed a number of other genes located on this chromosome that are likely to encode information important for formation and function of the male gametes. The Y chromosome was once thought to be responsible only for turning on sex determination towards the male pathway via the testis determinant gene Sry. However, subsequent studies revealed a number of other genes located on this chromosome that are likely to encode information important for formation and function of the male gametes. The goal of this project is to define the roles of certain Y encoded genes. More specifically, we seek to (1) unveil the function and mechanism of action of genes encoded on the Y chromosome long arm, (2) test whether testis determinant gene Sry plays a role in sperm function; (3) define the minimum Y gene complement that is sufficient to generate gametes capable of achieving successful fertilization. This will be done through the analysis of unique mouse models lacking various parts of the Y chromosome, and use of assisted reproduction technologies (ART): intracytoplasmic sperm injection (ICSI) and in vitro fertilization (IVF), in combination with molecular biology approaches. Deletions within the male specific region of the Y chromosome are a common genetic cause of spermatogenic failure, making men carrying affected Y chromosome a target group for infertility treatment via ART. Given the known and potential problems associated with the use of ART in humans, it is essential to continue efforts on identifying the underlying causes of infertility. Our work will advance understanding of the Y chromosome encoded genes role in spermatogenesis and sperm function and ultimately may help to combat infertility, a major health problem in U.S.A.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 Y染色体曾经被认为只负责通过睾丸决定基因Sry将性别决定转向男性途径。 然而，随后的研究揭示了位于该染色体上的许多其他基因，这些基因可能编码对雄配子的形成和功能重要的信息。 Y染色体曾经被认为只负责通过睾丸决定基因Sry将性别决定转向男性途径。 然而，随后的研究揭示了位于该染色体上的许多其他基因，这些基因可能编码对雄配子的形成和功能重要的信息。 该项目的目标是定义某些 Y 编码基因的作用。 更具体地说，我们试图（1）揭示Y染色体长臂上编码的基因的功能和作用机制，（2）测试睾丸决定基因Sry是否在精子功能中发挥作用； (3) 定义足以产生能够成功受精的配子的最小Y基因互补体。 这将通过分析缺乏 Y 染色体各个部分的独特小鼠模型，并使用辅助生殖技术 (ART)：胞浆内单精子注射 (ICSI) 和体外受精 (IVF)，并结合分子生物学方法来完成。 Y 染色体男性特定区域内的缺失是生精失败的常见遗传原因，这使得携带受影响 Y 染色体的男性成为通过 ART 治疗不孕症的目标群体。 鉴于与人类使用辅助生殖技术相关的已知和潜在问题，有必要继续努力找出不孕不育的根本原因。 我们的工作将增进对 Y 染色体编码基因在精子发生和精子功能中作用的了解，并最终可能有助于解决美国的一个主要健康问题不孕症。