Telomerase-Specific Adenoviral Imaging Systems for Detecting and Isolating CTCs

用于检测和分离 CTC 的端粒酶特异性腺病毒成像系统

基本信息

批准号：
8435342
负责人：
JIYUE ZHU
金额：
$ 7.19万
依托单位：
PENNSYLVANIA STATE UNIV HERSHEY MED CTR
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-03-01 至 2014-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8435342
关键词：
Address Adenoviruses Affect Antibodies Antigens CMV promoter Cancer Patient Cancer cell line Catalytic Domain Cell Adhesion Molecules Cell surface Cells Clinical Clinical Trials Colorectal Cancer Cytokeratin DNA Tumor Viruses Detection Devices Doxycycline Early Diagnosis Elements Epithelial Epithelial Cells Evaluation Event FDA approved Fibroblasts Future Gene Expression Regulation Generations Genes Heterogeneity Human Image Label Lead Magnetism Malignant Neoplasms Mesenchymal Methods Molecular Monitor Neoplasm Circulating Cells Neoplasm Metastasis Normal Cell Patients Principal Investigator Procedures Prognostic Marker Property Recombinants Reporting Research Research Personnel Solid Neoplasm Somatic Cell Stem cells System TERT gene Techniques Technology Telomerase Testing Tetanus Helper Peptide Tissues Training Tumor Stem Cells Viral Work base cancer cell cancer recurrence cancer type clinically relevant expectation metastatic colorectal minimally invasive neoplastic cell novel outcome forecast peripheral blood platform-independent promoter replication competent adenoviral vector treatment response tumor vector

项目摘要

DESCRIPTION (provided by applicant): Tumors shed their cells into the peripheral blood, generating metastases that confer their lethality. Circulating tumor cells (CTCs) have been detected in patients of many types of cancers, even those with no signs of clinically overt metastases. Because CTCs are accessible using minimally invasive procedures, the detection and characterization of CTCs are pivotal for early diagnosis, prognosis, and treatment monitoring, as well as for understanding of the molecular and cellular events that lead to metastases. Our objective is to establish a sensitive and reliable method for detecting and capturing CTCs of all tumor types, regardless of their tissue origins, in research and clinical settings. We hypothesize that the telomerase-selective adenoviruses specifically label cancer cells and provide a simple method for efficient CTC detection and isolation. Our specific aims are (1) to generate replication-competent adenoviral vectors with optimized hTERT promoters for selective labeling of viable cancer cells; (2) to generate and characterize a tet-regulatable telomerase-selective adenovirus for the detection and isolation of viable CTCs.

描述（由申请人提供）：肿瘤将其细胞脱落到周围血液中，产生赋予其致死性的转移酶。在许多类型的癌症患者中，即使是没有临床明显转移迹象的患者，也发现了循环肿瘤细胞（CTC）。由于CTC可以使用最小的侵入性程序访问，因此CTC的检测和表征对于早期诊断，预后和治疗监测是关键，以及了解导致转移酶的分子和细胞事件。我们的目标是在研究和临床环境中建立一种敏感且可靠的方法，用于检测和捕获所有肿瘤类型的CTC。我们假设端粒酶选择性腺病毒专门标记癌细胞，并为有效的CTC检测和分离提供了一种简单的方法。我们的具体目的是（1）用优化的HTERT启动子生成具有复制能力的腺病毒载体，以选择可行的癌细胞；（2）生成和表征可将可行CTC检测和分离的可tet端粒酶选择性腺病毒。