The role of Apobec3 enzymes in regulation of marginal zone B cells

Apobec3 酶在边缘区 B 细胞调节中的作用

• 批准号: 8564959
• 负责人: Anthony L Defranco
• 金额: $ 22.14万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8564959
• 关键词: Affect; Antibodies; Antigens; Applications Grants; B-Lymphocytes; Biological; Blood-Borne Pathogens; Bone Marrow; Cell Count; Cell Separation; Cells; Complementary DNA; DNA; Development; Employee Strikes; Environment; Enzymes; Exogenous Factors; Family; Gene Expression Profile; Genes; Genome; Goals; Health; Human; Human Genome; Immune system; Immunoglobulin M; Inflammation; Insertional Mutagenesis; Interferon Receptor; Interferons; Isoenzymes; Lymphocyte; Malignant Neoplasms; Malpighian corpuscles; Mediating; Mus; Population; Proteins; Regulation; Retroelements; Retrotransposon; Retroviridae; Role; Spleen; Splenic Red Pulp; T-Lymphocyte; Testing; Transgenic Mice; deep sequencing; design; insight; macrophage; mouse genome; research study; response

DESCRIPTION (provided by applicant): The role of Apobec3 enzymes in regulation of marginal zone B cells The Apobec3 enzymes are intensely studied restriction factors of exogenous and endogenous retroelements (retroviruses and retrotransposons). Endogenous retroelements make up almost half of the mouse and human genomes-many of them somatically active. They often are interspersed within cellular genes and are thus transcribed along with cellular genes. Unrestricted retroelements can cause inflammation and, via insertional mutagenesis, cancer, but the innate and adaptive immune systems keep most of them in check. We found that Apobec3 enzymes decrease, in an activity-graded manner, the number of marginal zone B cells of mice, but not the number of follicular B cells or T cells. We, therefore, suggest that endogenous retroelements drive the development and/or expansion of the marginal zone B-cell population. In this grant application, we propose to test whether the retroelements activity is intrinsic to the marginal zone B cells, and to identify the retroelements

描述（由申请人提供）：APOBEC3酶在边缘区B细胞调节中的作用APOBEC3酶是对外源性和内源性恢复元素（逆转录病毒和后跨跨植物）的深入研究的限制因子。内源性追溯元素几乎构成了小鼠和人类基因组的一半，它们具有体积活跃。它们通常散布在细胞基因中，因此与细胞基因一起转录。不受限制的恢复元素可能会引起炎症，并通过插入诱变，癌症，但先天和适应性免疫系统使大多数人都可以控制。我们发现，APOBEC3酶以活性级别的小鼠边缘区B细胞数量减少，而不是卵泡B细胞或T细胞的数量。因此，我们建议内源性追溯元素驱动边缘区B细胞种群的发展和/或扩展。在此赠款应用中，我们建议测试恢复元素活性是否与边缘区B细胞固有，并识别重新元素