INTEGRATIVE BIOLOGY

综合生物学

基本信息

批准号：
8474134
负责人：
LENARD M LICHTENBERGER
金额：
$ 16.93万
依托单位：
BAYLOR COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-04-15 至 2018-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8474134
关键词：
Acute Animal Model Animals Biological Markers Biological Models Biology Caring Cell Culture Techniques Cell Line Cell Separation Cells Chronic Collaborations Databases Deformity Derivation procedure Development Digestive System Disorders Enrollment Experimental Designs Faculty Gastrointestinal Diseases Gastrointestinal Motility Gastrointestinal Physiology Gastrointestinal Surgical Procedures Gastrointestinal tract structure Genes Goals Growth Guidelines Hand Hepatocyte Human Image Imaging Techniques In Vitro Individual Infection Injury Institutional Review Boards Instruction Intestines Kupffer Cells Laboratories Laboratory Animals Measurement Medical center Methods Modeling Molecular Monitor Movement Mus Muscle function Operative Surgical Procedures Organoids Pathology Performance Pharmaceutical Preparations Pharmacology Physiologic Monitoring Physiological Preparation Procedures Property Protocols documentation Rattus Research Research Personnel Resources Rodent Role Schedule Services Smooth Muscle Stem cells Structure System Techniques Texas Tissues Tracer Training Transgenic Animals Transgenic Organisms United States National Institutes of Health Work animal model development animal tissue design experience gastrointestinal gastrointestinal function gastrointestinal infection human tissue in vitro Model in vivo member microorganism research study response trafficking

项目摘要

PROJECT SUMMARY (See instructions): The Integrative Biology Core provides training and service to the members ofthe Texas Medical Center Digestive Diseases Research Core Center (DDC) and their research staff in: 1) animal surgery of the gastrointestinal (Gl) tract; 2) physiological measurements of Gl properties utilizing both in vivo and in vitro systems; 3) the development of animal models of digestive diseases; 4) cell isolation; 5) whole animal imaging techniques to non-invasively detect functional and anatomical abnormalities, and to monitor the traffic and distribution of biomarkers, drugs and microorganisms. Drs. Lichtenberger and Uray at UTHSC have expertise in the field of Gl physiology and pharmacology, specializing in the in vivo and in vitro models of Gl secretion, barrier function and Gl smooth muscle function, and have developed a number of animal models in their laboratories to study pathophysiological mechanisms of digestive diseases. Dr. Smith, a Collaborator, will bring expertise in animal surgery to the Core and provide assistance and training to members ofthe Center whose research requires animal handling and/or surgery and will provide assistance in issues related to IRB approval for research on laboratory animals. Dr. Thevananther will provide a valuable serve to members of the DDC who require isolated rodent hepatocytes and Kupffer cells for culture or other in vitro studies, with Kupffer cell isolation being a new service. We have also added a new service to assist DDC members in the growth and study Gl organoids in culture. Another service offered to members of the DDC will be to provide assistance with whole animal imaging to non-invasively detect alterations in structure and function, and to monitor the movement of molecular tracers, which has been used successfully to monitor Gl motility and barrier properties. The Integrative Biology Core aims to assist the members ofthe Center in the development of the required techniques utilizing laboratory animals and tissues to accomplish their research goals. In addition to the Directors, the services of an experienced Animal Physiological & Surgical Technician will provide hands on training of members ofthe DDC in accordance to a prioritization schedule to assure the development of junior investigators. The staff of the Integrative Biology Core will also serve to facilitate interactions and collaborations between members ofthe Center.

项目摘要（请参阅说明）：综合生物学核心为德克萨斯医学中心消化疾病研究中心（DDC）及其研究人员提供培训和服务：1）胃肠道（GL）动物手术； 2）利用体内和体外系统的GL性质的生理测量； 3）消化疾病动物模型的发展； 4）细胞分离； 5）整个动物成像技术可用于非侵入性检测功能和解剖异常，并监测生物标志物，药物和微生物的交通和分布。博士。 UTHSC的Lichtenberger和Uray在GL生理学和药理学领域具有专业知识，专门研究体内和体外的GL分泌，屏障功能和GL平滑肌功能，并在其实验室中开发了许多动物模型来研究消化疾病的病理生理机制。合作者Smith博士将把动物外科手术方面的专业知识带到核心，并向中心的成员提供帮助和培训，其研究需要动物处理和/或手术，并将在与IRB批准有关实验室动物研究有关的问题中提供帮助。 Thevananther博士将为需要孤立的啮齿动物肝细胞和库普弗细胞进行培养或其他体外研究的DDC成员提供宝贵的服务，而Kupffer细胞分离是一种新服务。我们还添加了一项新服务，以帮助DDC成员在培养中的生长和研究类器官。提供给DDC成员的另一项服务是为全动物成像提供帮助，以非侵入性检测结构和功能的改变，并监测分子示踪剂的运动，该分子示踪剂已成功地用于监测GL运动和屏障性能。综合生物学核心旨在帮助中心成员利用实验室动物和组织来实现其研究目标的开发。除董事外，经验丰富的动物生理和外科技术人员的服务还将根据确保开发初级调查人员的发展时间表，以培训DDC成员的培训。综合生物学核心的工作人员还将有助于促进中心成员之间的互动和合作。