Metabolomics Core

代谢组学核心

基本信息

批准号：
8517666
负责人：
GERALD I SHULMAN
金额：
$ 19.41万
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-07-01 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8517666
关键词：
1,2-diacylglycerol Accounting Acyl Coenzyme A Address Automation Biochemistry Biological Assay Blood Plasma Volume Calibration Carbon Ceramides Charge Chemistry Communities Complex Complications of Diabetes Mellitus Creatinine Development Diabetes Mellitus Diabetic mouse Diglycerides Divalent Cations Engineering Ensure Esters Fatty Acids Fee-for-Service Plans Funding Gene Expression Glucose Plasma Concentration Goals High Pressure Liquid Chromatography Hormones Human Resources Instruction Isotopes Kinetics Laboratories Length Lipids Liver Function Tests Maintenance Measurement Measures Metabolic Metabolic Diseases Methods Modeling Molecular Mus Nonesterified Fatty Acids Obesity Organ Phenotype Physiology Plasma Policies Progress Reports Protocols documentation Quality Control Radio Renal clearance function Research Research Personnel Resources Sampling Savings Serum Services Stable Isotope Labeling System Systems Analysis Testing Tissue Sample Tissues Tracer Training Transgenic Mice United States National Institutes of Health Universities Urine Vendor Washington Work base carbohydrate metabolism cytokine design enzyme activity experience flexibility glucose metabolism in vivo instrument instrumentation lipid metabolism liquid chromatography mass spectrometry meetings metabolomics mouse model programs protein metabolism research and development research study response stable isotope

项目摘要

.PROJECT SUMMARY (See instructions): The Metabolomics Core provides a facility to determine the phenotype of metabolite and hormone profiles of transgenic mouse models of diabetes and its complications. The services provided by the Metabolomics Core include the extraction, purification, derivatization, and instrumental analysis of metabolites, hormones, and selected enzyme activities from tissues and plasma. The methods have been scaled to account for the minimal amounts of tissues (-10 to 25 mg, and plasma volumes of - 5 to 25 pi) that can be provided by investigators.. The facility is equipped with an array of state-of-the-art instrumentation, including GC-MS, LC/MS/MS, HPLC, and NMR, providing the flexibility and resources for analyses of metabolite concentrations as well as sotopic enrichments. Metabolic panels of serum, plasma, and urine, including Chem 7, liver function tests lipid profiles, and divalent cations, are obtained using the COBAS MIRA system. We have optimized the COBAS Mira analytical system for analysis of mouse samples such that required volumes average -20 pi of serum for any combination of 4 tests. These plasma chemistry assays fill a need with very high demand for mouse studies, as reflected in our work flow over the last funding period. GC/MS and LC/MS/MS analyses provide complete profiles of chain length distribution of fatty acid metabolites, included free fatty acids, fatty acyl-CoA esters, diacylglycerols, ceramides. Other LC/MS/MS that are offered and are in high-demand include intermediates of glucose metabolism for metabolic flux measurements, and creatinines for renal clearance. The Metabolomics Core is a resource lab for the analysis of samples generated during the course of experiments of the Integrative Physiology Core at the Yale MMPC, and also provides the same array of assays to other outside investigators to assist in phenotyping of transgenic mouse models of diabetes Samples are analyzed on a fee-for-service basis with the charge per assay based on policy determined by the National MMPC Executive Committee, designed to provide substantial savings to NIH-funded users compared to commercial vendors. The Core has an active Research and Development Program to develop new analytical protocols to address new and exciting developments in the biochemistry of diabetes, obesity, and associated complications

.项目摘要（参见说明）：代谢组学核心提供了确定糖尿病及其并发症转基因小鼠模型的代谢物表型和激素谱的工具。代谢组学核心提供的服务包括从组织和血浆中提取、纯化、衍生化和代谢物、激素和选定酶活性的仪器分析。这些方法已按比例调整，以考虑研究人员可以提供的最小组织量（-10 至 25 mg，血浆体积 - 5 至 25 pi）。该设施配备了一系列最先进的仪器，包括 GC-MS、LC/MS/MS、HPLC 和 NMR，为代谢物浓度和同位素富集分析提供了灵活性和资源。使用 COBAS MIRA 系统获得血清、血浆和尿液的代谢组，包括 Chem 7、肝功能测试脂质谱和二价阳离子。我们优化了用于分析小鼠样品的 COBAS Mira 分析系统，使得 4 项测试的任意组合所需的血清体积平均为 -20 pi。这些血浆化学测定满足了对小鼠研究的非常高的需求，正如我们在上一个资助期间的工作流程所反映的那样。 GC/MS 和 LC/MS/MS 分析提供了脂肪酸代谢物链长分布的完整概况，包括游离脂肪酸、脂肪酰辅酶 A 酯、二酰甘油、神经酰胺。其他提供且需求量很大的 LC/MS/MS 包括用于代谢通量测量的葡萄糖代谢中间体，以及用于肾脏清除率的肌酐。代谢组学核心是一个资源实验室，用于分析耶鲁大学 MMPC 综合生理学核心实验过程中产生的样本，并且还为其他外部研究人员提供相同的一系列检测，以协助糖尿病转基因小鼠模型的表型分析样品分析采用按服务付费的方式，每次检测的费用根据国家 MMPC 执行委员会确定的政策进行，旨在为 NIH 资助的用户提供与商业供应商相比可观的节省。该核心有一个积极的研究和开发计划，以开发新的分析方案，以解决糖尿病、肥胖症和相关并发症的生物化学方面令人兴奋的新进展