Alternative treatments for disc degeneration: Effects on matrix homeostasis

椎间盘退变的替代治疗：对基质稳态的影响

基本信息

批准号：
8411092
负责人：
Gwendolyn A Sowa
金额：
$ 12.49万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-05-01 至 2014-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8411092
关键词：
1 year old Acids Address Affect Age Aging-Related Process Alternative Therapies Animal Model Animals Biochemical Pathway Biological Assay Biological Availability Biological Models Biomechanics Cartilage Catabolism Cell Culture Techniques Cells Centers for Disease Control and Prevention (U.S.)Chondroitin Clinical Research Clinical Treatment Collagen Data Development Diet Dinoprostone Environment Equilibrium Extracellular Matrix Future Gene Expression Gene Expression Profiling Glucosamine Glucosamine Sulfate Goals Height Histology Homeostasis Human In Vitro Inflammation Inflammatory Instruction Intervention Intervertebral disc structure Investigation Laboratories Lead Low Back Pain Magnetic Resonance Imaging Measurement Measures Mechanical Stimulation Mechanical Stress Mechanics Mediator of activation protein Metabolism Metalloproteases Modeling Molecular Motion Movement Nitric Oxide Nutritional Omega-3 Fatty Acids Operative Surgical Procedures Oral Oryctolagus cuniculus Outcome Pathway interactions Patients Performance Pharmaceutical Preparations Physiological Process Proteoglycan Regulatory Pathway Reporting Research Robot Role Staging Stimulus Stress Structural Genes System Techniques Testing Tissues Training Translating Translations Treatment Protocols Vertebral column Water Whole Organism Work Yoga aggrecan aggrecanase alternative treatment base chronic back pain cyclooxygenase 2 design dietary supplements experience human NOS2A protein in vivo in vivo Model indexing indium arsenide inhibitor/antagonist intervertebral disk degeneration kinematics novel nucleus pulposus prevent programs protective effect response simulation therapeutic target

项目摘要

Intervertebral disc degeneration is characterized by progressive loss of disc height related to loss of water content and proteoglycans. Key mechanisms involved in this process include inflammatory and mechanical stress. It is biologically plausible that CAM therapies, such as yoga or nutritional supplements, may exert their beneficial actions on the spine through these pathways. The fundamental goals of this research are to investigate the mechanisms behind the effects of motion based therapies, such as yoga, and oral supplements, including glusosamine, chondroitin, and omega-3 fatty acids, on matrix balance in the intervertebral disc through use of both in vitro and in vivo systems. The immediate impact of this work is in demonstrating the biologic effects of mechanical loading and nutritional supplements on the intervertebral disc using cell culture and animal model systems. This will provide us with a powerful experimental platform with which we can test the effect of various additional loading paradigms as well as nutritional supplements on disc matrix homeostasis. Upon elucidation of the effected biochemical pathways, future studies will correlate the model systems to human spine kinematics relevant to CAM motion based therapies. This will result in the ability to translate these findings into more mechanistically directed clinical studies and rationally targeted therapeutics, which represents the long term goal of the candidate. This work and the expertise gained through the proposed training plan will serve as initial building blocks for a research program in which CAM questions can be addressed at the molecular, tissue, and whole organism level. RELEVANCE (See instructions): In a CDC report from 2004, low back pain was identified as the most common condition for which patients sought alternative treatments such as yoga and oral supplements. To utilize these interventions most effectively, it is critical to understand how these therapies are facilitating their effects. The goals of this research are to understand the effects of motion based therapies, such as yoga, and oral supplements, including glusosamine, chondroitin, and omega-3 fatty acids, on the cartilage of the spine.

椎间盘退化的特征是椎间盘高度的逐渐丧失与水的流失有关内容和蛋白聚糖。此过程中涉及的关键机制包括炎症和机械机制压力。在生物学上，CAM疗法（例如瑜伽或营养补充剂）可能会发挥作用是合理的他们通过这些途径对脊柱的有益行动。这项研究的基本目标是研究基于运动疗法的作用（例如瑜伽和口服）背后的机制补充剂，包括glusosamine，软骨素和omega-3脂肪酸，在基质中的平衡椎间盘通过使用体外和体内系统。这项工作的直接影响是证明机械负荷和营养补充剂对椎间盘的生物学作用使用细胞培养和动物模型系统的光盘。这将为我们提供一个强大的实验平台我们可以通过其中测试各种额外的加载范式以及营养补充剂的效果在椎间盘基质稳态上。阐明了受影响的生化途径，未来的研究将将模型系统与与基于CAM运动的疗法相关的人脊柱运动学相关联。这会导致将这些发现转化为更机械定向的临床研究的能力并合理地有针对性的治疗学，代表了候选人的长期目标。这项工作和专业知识通过拟议的培训计划获得的将作为研究计划的初始基础 CAM问题可以在分子，组织和整个生物体水平上解决。相关性（请参阅说明）：在2004年的CDC报告中，腰痛被确定为患者最常见的病情寻求其他治疗方法，例如瑜伽和口服补充剂。大多数使用这些干预措施有效地，了解这些疗法如何促进其作用至关重要。目标的目标研究是为了了解基于运动的疗法的影响，例如瑜伽和口服补充剂，在脊柱软骨上，包括胶质胺，软骨素和omega-3脂肪酸。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Part 1: Dual-tuned proton/sodium magnetic resonance imaging of the lumbar spine in a rabbit model.

DOI：
10.1097/brs.0b013e318259ee98
发表时间：
2012-08-15
期刊：
Spine
影响因子：
3
作者：
Moon CH;Kim JH;Jacobs L;Zhao T;Sowa G;Vo N;Kang J;Bae KT
通讯作者：
Bae KT

Part 2: Quantitative proton T2 and sodium magnetic resonance imaging to assess intervertebral disc degeneration in a rabbit model.

DOI：
10.1097/brs.0b013e3182583447
发表时间：
2012-08-15
期刊：
Spine
影响因子：
3
作者：
Moon CH;Jacobs L;Kim JH;Sowa G;Vo N;Kang J;Bae KT
通讯作者：
Bae KT

NSAID use in intervertebral disc degeneration: what are the effects on matrix homeostasis in vivo?

NSAID 在椎间盘退变中的应用：对体内基质稳态有何影响？

DOI：
10.1016/j.spinee.2017.04.012
发表时间：
2017
期刊：
The spine journal : official journal of the North American Spine Society
影响因子：
0
作者：
Vaudreuil,Nicholas;Kadow,Tiffany;Yurube,Takashi;Hartman,Robert;Ngo,Kevin;Dong,Qing;Pohl,Pedro;Coelho,JPaulo;Kang,James;Vo,Nam;Sowa,Gwendolyn
通讯作者：
Sowa,Gwendolyn

The effects of glucosamine sulfate on intervertebral disc annulus fibrosus cells in vitro.

硫酸氨基葡萄糖对体外椎间盘纤维环细胞的影响。

DOI：
10.1016/j.spinee.2013.11.058
发表时间：
2015
期刊：
The spine journal : official journal of the North American Spine Society
影响因子：
0
作者：
Sowa,GwendolynA;Coelho,JPaulo;Jacobs,LloydineJ;Komperda,Kasey;Sherry,Nora;Vo,NamV;Preuss,HarryG;Balk,JudithL;Kang,JameD
通讯作者：
Kang,JameD

Glucosamine supplementation demonstrates a negative effect on intervertebral disc matrix in an animal model of disc degeneration.

DOI：
10.1097/brs.0b013e318286b31e
发表时间：
2013-05-20
期刊：
Spine
影响因子：
3
作者：
Jacobs L;Vo N;Coelho JP;Dong Q;Bechara B;Woods B;Hempen E;Hartman R;Preuss H;Balk J;Kang J;Sowa G
通讯作者：
Sowa G