Biology and Bioengineering Core

生物学与生物工程核心

基本信息

批准号：
8479417
负责人：
scott john
金额：
$ 27.5万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-08-10 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8479417
关键词：
Adult Alloys Anatomy Animals Area Arrhythmia Autopsy Biological Biology Biomedical Engineering Cardiac Cardiovascular system Cells Chimera organism Clinical Clinical Research Collaborations Computers Coupling DNA Development Dissection Doctor of Philosophy Electron Microscopy Electronics Electrons Engineering Equipment Evaluation Fluorescent Dyes Future Gene Transfer Generations Genes Genetic Techniques Head Health Sciences Histocytochemistry Histology Histopathology Housing Human Resources Image Image Analysis In Situ Hybridization Infection Laboratories Laboratory Research Lasers Life Light Microscope Maintenance Maps Measures Mechanics Medical center Medicine Messenger RNA Metals Methodology Microscope Microscopic Microscopy Molecular Molecular Biology Molecular Biology Techniques Monitor Muscle Cells Northern Blotting Optics Oryctolagus cuniculus Pathologist Pathology Photography Physicians Plastics Play Preparation Procedures Process Production Proteins Publications Research Research Personnel Resources Role Running Scanning Scanning Electron Microscopy Scientist Services Site Slide Small Interfering RNA Specimen Speed Staining method Stains Structure Supervision System Technical Expertise Techniques Technology Testing Tissue Sample Tissue Stains Tissues Ventricular Viral Viral Genes Viral Vector Western Blotting Work cryostat design design and construction experience expression vector fluorescence imaging fluorescence microscope gel electrophoresis gene transfer vector human tissue immunocytochemistry improved in vivo insertion/deletion mutation instrument instrumentation medical schools member morphometry mutant operation patch clamp square foot theories tissue culture tissue processing tissue/cell culture tool vector

项目摘要

Laboratory and Bioengineering Core B, directed by Scott A. John, PhD, will provide support to PPG Projects in the following areas: 1. Molecular biology and gene transfer vectors 2. Pathology and histology 3. High speed laser confocal fluorescence imaging 4. Customized bio-instrumentation fabrication and maintenance The molecular biology component will be conducted in the Cardiovascular Research Laboratory in the MacDonald Research Building, under the direct supervision of Dr. Scott John, who leads the molecular biology group in Dr. Weiss's laboratory. Dr. Yibin Wang, an expert in gene vector development, will provide advice and oversight in this area, in collaboration with Dr. John. The histopathology component will be conducted in the Cardiovascular Research Laboratory in the MacDonald Research Building, performed by Fuhua Chen, MD, a cardiovascular physiologist, under the direct oversight of Michael Fishbein, MD, an internationally recognized cardiovascular pathologist and chief of autopsy pathology at UCLA Medical Center, who has a longstanding interaction with the PPG investigators. The high speed laser confocal fluorescence imaging in live cells will be overseen by Joshua Goldhaber, MD, a cardiovascular physiologist (and cardiologist) who is an expert in cardiac excitation contraction coupling. The bioengineering component will be headed by John Parker who has provided technical expertise for many of the PPG investigators in the past. He runs the Biomedical Instrumentation Facility, housed in the UCLA Center for the Health Sciences Building, which is readily accessible to the PPG investigators. This facility has extensive experience providing biomedical instrumentation services for scientists and physicians, having designed and constructed many of the instruments currently being utilized by the PPG investigators.

由Scott A. John博士执导的实验室和生物工程核心B将为以下领域的PPG项目提供支持： 1。分子生物学和基因转移载体 2。病理学和组织学 3。高速激光共聚焦荧光成像 4。定制的生物启示制造和维护分子生物学组成部分将在麦克唐纳研究大楼的心血管研究实验室中进行，在斯科特·约翰（Scott John）博士的直接监督下。基因矢量发展专家Yibin Wang博士将与John博士合作，在该领域提供建议和监督。组织病理学组件将在麦克唐纳研究大楼的心血管研究实验室中进行，由心血管生理学家富胡岛陈（Fuhua Chen）在迈克尔·菲什贝因（Michael Fishbein）的直接监督下进行，该医学博士是一名国际性心血管病理学家兼自动病理学家迈克尔·菲什贝因（Michael Fishbein），在UCLA医疗中心，他的经常性病理学家的长期互动，该中心是一个长期以来的PPP互动。活细胞中的高速激光共聚焦荧光成像将由约书亚监督医学博士Goldhaber是心血管生理学家（和心脏病专家），他是心脏激发收缩耦合的专家。生物工程组件将由约翰·帕克（John Parker）领导，约翰·帕克（John Parker）过去曾为许多PPG调查人员提供技术专长。他经营着位于加州大学洛杉矶分校卫生科学大楼中心的生物医学仪器设施，PPG调查人员很容易接触。该设施具有丰富的经验，可为科学家和医生提供生物医学仪器服务，设计和构建了PPG调查人员目前正在使用的许多工具。