Tissue Engineering of New Hair Formation

新毛发形成的组织工程

• 批准号: 8530017
• 负责人: Cheng-Ming Chuong
• 金额: $ 33.24万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-21 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8530017
• 关键词: Adult; Alopecia; Appearance; Architecture; Area; Artificial skin; Beds; Biological Assay; Burn Trauma; Burn injury; Cells; Cicatrix; Clinical; Complex; Cosmetics; Cyst; Defect; Dermal; Diffusion; Environment; Feathers; Future; Generations; Goals; Growth; Hair; Hair follicle structure; Hair shaft structure; Human; Infection; Learning; Length; Lentivirus Vector; Measures; Methods; Modeling; Modification; Molecular; Mus; Newborn Infant; Organ; Patients; Pattern; Pattern Formation; Primordium; Procedures; Process; Production; Protocols documentation; Reaction; Regulation; Research; Running; Sebaceous Glands; Shapes; Skin; Source; Stem cells; Sterile coverings; Surface; Sweat Glands; Technology; Testing; Therapeutic; Thick; Tissue Engineering; Tissues; Translating; Trauma; United States; Water; appendage; base; cellular engineering; clinical application; density; design; experience; flexibility; high throughput screening; improved; novel; parent grant; public health relevance; reconstitution; regenerative; screening; success; technology development; wound

DESCRIPTION (provided by applicant): The objective of this proposal is to develop reconstituted skin that can form new hairs from dissociated stem cells. Progress has been made in producing hair follicles using dissociated multi-potential dermal and epidermal stem cells. However, couple gaps need to be filled before significant progress in clinical applications can be made. Recently, we have improved significantly the previous assays by Lichti and Stenn into a simpler hair forming assay with clinically proper appearance and potential for a high throughput assay. Our general hypothesis is that multi-potent skin stem cells can be generated by molecular reprogramming, and that arrangement and patterning of hair primordia can be regulated by environmental conditions. With the newly developed "planar hair forming procedure", we are able to apply our expertise in feather pattern formation to regulate the number and size of hair primordia. We will also apply our recent experience on macro-environment regulation of hair stem cells to improve the efficiency of hair formation. Recent success of molecular re-programmig in other organs has inspired excitement. This simple assay will allow us to do high throughput analyses to identify molecules or combinations of molecules important to reprogram adult skin cells into hair forming dermal and epidermal cells. The development of this technology will bring new hope to those who suffer from severe wounds and alopecia. PUBLIC HEALTH RELEVANCE: Currently, over 100,000 patients are treated for burn injury in the United States per year, and burns can cause lasting cosmetic and functional defects to visible areas on the skin. Here we propose to develop a novel planar hair forming protocol which integrates progress from our parent grant and others hair research and may bring new hope to those who suffer from severe wounds, and alopecia.

描述（由申请人提供）：该提案的目的是开发可以从分离的干细胞形成新毛发的重建皮肤。使用分离的多能真皮和表皮干细胞生产毛囊已取得进展。然而，在临床应用取得重大进展之前，还需要填补一些空白。最近，我们对 Lichti 和 Stenn 之前的测定方法进行了显着改进，使其成为一种更简单的毛发形成测定方法，具有临床上正确的外观和高通量测定的潜力。我们的一般假设是，多能皮肤干细胞可以通过分子重编程产生，并且毛发原基的排列和图案可以通过环境条件来调节。通过新开发的“平面毛发形成程序”，我们能够运用我们在羽毛图案形成方面的专业知识来调节毛发原基的数量和大小。我们还将应用最近在毛发干细胞宏观环境调控方面的经验来提高毛发形成的效率。最近在其他器官中进行分子重新编程的成功激发了人们的兴奋。这种简单的测定将使我们能够进行高通量分析，以确定对将成人皮肤细胞重新编程为毛发形成真皮和表皮细胞很重要的分子或分子组合。这项技术的发展将为那些遭受严重创伤和脱发的人们带来新的希望。 公共健康相关性：目前，美国每年有超过 100,000 名烧伤患者接受治疗，烧伤会对皮肤可见区域造成持久的外观和功能缺陷。在这里，我们建议开发一种新颖的平面头发形成方案，该方案整合了我们家长资助和其他头发研究的进展，可能为那些遭受严重伤口和脱发的人带来新的希望。