In Vivo Study Support Core

体内研究支持核心

• 批准号: 8568008
• 负责人: DONNA M. BOULEY
• 金额: $ 21.09万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8568008
• 关键词: Ablation; Acute; Aftercare; Animal Model; Appearance; Cancer Intervention; Cell Death; Chemicals; Computer software; Cryosurgery; Data; Development; Devices; Dimensions; Drug Formulations; Feedback; Freezing; Goals; Histologic; Histology; Histopathology; Image; Individual; Lesion; Location; Magnetic Resonance Imaging; Maps; Measurement; Methods; Microscopic; Monitor; Outcome Measure; Paraffin Embedding; Pathologic; Pathology; Phase; Preparation; Procedures; Process; Protocols documentation; Radiology Specialty; Research; Slice; Slide; Specimen; Staining method; Stains; Structure; Techniques; Temperature; Testing; Time; Tissue Fixation; Tissue Viability; Tissues; Weight; Work; base; cancer therapy; clinical application; design; digital; image registration; imaging modality; improved; in vivo; minimally invasive; research study; sample fixation; statistics; success; tool

The In Vivo Study Support Core will develop and test the methods (imaging and histopathology) that will be used in all 5 projects to evaluate the in vivo functionality and accuracy of the minimally invasive cancer treatments developed in this PO1. Analysis of the macroscopic and microscopic pathology in post-treatment target tissues will be performed by this core in order to provide the critical feedback - paramount to the success of these minimally invasive clinical applications. The core will optimize MRI post - treatment imaging, and develop methods for registration between MR and histopathology images. For quality correlation of gross as well as macro, microscopic, and MR Images, this core will customize target-tissue specific lesion sampling, fixation, processing, embedding, slide preparation, and interpretation of the histopathology for all animal models used in this PO1. Furthermore, accuracy of correlation between MRI and histopathology will be accomplished by placement of fiducial markers in target tissues, digitization of histological slides, and registration of the MR images with digital gross and microscopic pathology images. These techniques will enable correction of lesion measurements due to tissue fixation shrinkage, and distortion of tissues due to manipulation and processing. In addition, we will incorporate special chemical and immunohistochemical stains, as needed, to detect markers of acute cell death and, degrees of tissue damage, in order to improve interpretation of thermal lesion appearance via the various acute post-treatment MR imaging modalities. Statistical support will also be provided by this core. The four Specific Aims of Core A are to: Aim 1: Provide Imaging for Immediate Post-Ablation Assessment of Thermal and Cryoablation Lesions. Aim 2: Provide Methods for Correlating MR Imaging and Pathology Slides. Aim 3: Provide Methods for Enhanced Pathologic Analysis of Thermal and Cryoablation Lesions. Aim 4: Provide Statistical Support for Data Generated in Projects 1-5 and Cores A and B.

体内研究支持核心将开发和测试方法（成像和组织病理学）， 用于所有 5 个项目，以评估微创的体内功能和准确性 本 PO1 中开发的癌症治疗方法。宏观和微观病理学分析 治疗后目标组织将由该核心执行，以提供关键反馈 - 对于这些微创临床应用的成功至关重要。核心将优化 MRI 治疗后成像，并开发 MR 和组织病理学图像之间的配准方法。 对于总体以及宏观、微观和 MR 图像的质量相关性，该核心将定制 目标组织特异性病变取样、固定、处理、包埋、载玻片制备和 本 PO1 中使用的所有动物模型的组织病理学解释。此外，准确度 MRI 和组织病理学之间的相关性将通过在 目标组织、组织学切片的数字化以及 MR 图像与数字粗体和 显微病理图像。这些技术将能够校正由于以下原因造成的病变测量 由于操作和加工而导致的组织固定收缩和变形。此外，我们将 根据需要结合特殊化学和免疫组织化学染色剂，以检测急性细胞标记物 死亡和组织损伤程度，以改善对热损伤外观的解释 各种急性治疗后 MR 成像方式。本次活动还将提供统计支持 核。 核心 A 的四个具体目标是： 目标 1：为热消融和冷冻消融病变的消融后立即评估提供影像。 目标 2：提供关联 MR 成像和病理切片的方法。 目标 3：提供热消融和冷冻消融病变的强化病理分析方法。 目标 4：为项目 1-5 以及核心 A 和 B 中生成的数据提供统计支持。