BD FACSAria System

BD FACSAria 系统

• 批准号: 8447164
• 负责人: Edward F Srour
• 金额: $ 51.57万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-20 至 2014-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447164
• 关键词: 13 year old; Agreement; Calendar; Cancer Center; Cell Separation; Cells; Containment; Cytometry; Environment; Extramural Activities; Fee-for-Service Plans; Flow Cytometry; Funding; Grant; Guidelines; Health Services Accessibility; Hour; Indiana; International; Investigation; Laboratories; Lasers; Policies; Productivity; Research; Research Personnel; Resources; Sampling; Science; Secure; Services; Societies; System; Technology; United States National Institutes of Health; Universities; base; cell type; institutional biosafety committee; instrument; medical schools

DESCRIPTION (provided by applicant): The Flow Cytometry Resource Facility (FCRF) at the Indiana University Simon Cancer Center (IUSCC) is a centralized shared facility providing multiple flow cytometric services to over 100 investigators at the Indiana University School of Medicine (IUSM). The FCRF is a strictly research-dedicated, fee-for-service core laboratory with flow cytometric cell sorting being a major service provided by this facility. The FCRF was established in 1985 and has been part of the Indiana University Simon Cancer Center since 1992. During Calendar-Year 2010, forty-four investigators used over 1600 hours of cell sorting and in the last 7 years, the number of investigators using cell sorting services at the FCRF ranged between 41 and 53 per year. The majority of these investigators hold federally funded grants (primarily R01, P01 and R21 NIH grants and similar DoD grants). Many of the cell samples subjected to cell sorting by different users are classified as BSL2 or BSL3 and the handling of these cells should therefore follow guidelines established by the Institutional Biosafety Committee, the International Society of Analytical Cytometry Biosafety Guidelines, and the developing NIH-wide standards for biosafety in cell sorting laboratories. At present, one of the 3 sorters in the FCRF is a 13-year old FACSVantage SE that cannot be maintained under a service agreement anymore and that cannot be reconfigured to comply with any of the new biocontainment requirements for the safe handling of biohazardous materials. We are therefore requesting funds to acquire a Special Becton Dickinson FACSAria System for cell sorting composed of five lasers and contained completely within a BSL2/BSL3 biosafety cabinet. Our justification of need for this instrument is based on three critical necessities. First, BD does no service our FACSVantage SE anymore. In fact, since the original submission of this application, this instrument has been technically out of commission. Second, the evolving policies on biosafety levels, containment, and practices in flow cytometry by many governing bodies require the use of cell sorters with BSL2/BSL3 containment systems with many of the cell types subjected to cell sorting at the FCRF and which cannot be accomplished by the FACSVantage SE. Third, technological advancements in flow cytometry in general and cell sorting in particular make the FACSVantage SE a rather ineffective and inefficient cell sorter for most users wishing to apply state-of-the-art technologies in their research. These hard realities are at present creating a considerable strain on the availability of services provided by the FCRF for investigators and are beginning in some cases to hinder their productivity and competitiveness in securing extramural funding for their research. Acquisition of this new cell sorter should allow almost fifty investigators at the IUSM to conduct their research for years to come within a safe and compliant biocontainment environment and to benefit from state-of-the-art technologies that promote first class investigations and enhance cutting edge science.

描述（由申请人提供）：印第安纳大学西蒙癌症中心（IUSCC）的流式细胞仪资源设施（FCRF）是一家集中式共享设施，为印第安纳大学医学院（IUSM）的100多名研究人员提供多个流式细胞仪服务。 FCRF是一项严格研究的，用于支出费用的核心实验室，流式细胞仪分类是该设施提供的主要服务。 FCRF成立于1985年，自1992年以来一直是印第安纳大学西蒙癌症中心的一部分。在2010年日历年期间，四十四名研究人员使用了1600个小时的细胞分类，在过去的7年中，使用FCRF的细胞分类服务的研究人员数量在FCRF中为41至53范围。这些研究人员中的大多数持有联邦资助的赠款（主要是R01，P01和R21 NIH赠款以及类似的DOD赠款）。许多受不同用户经过细胞分类的细胞样品被归类为BSL2或BSL3，因此，应遵循机构生物安全委员会，国际分析细胞仪生物安全指南的指南，以及在细胞分类实验室中发展的NIH范围生物保护标准。目前，FCRF中的3个划者之一是13岁的FACSVantage SE，无法在服务协议下维护，并且不能重新配置以符合任何新的生物内保护要求，以安全处理生物危害材料。因此，我们要求资金购买特殊的Becton Dickinson Facsaria系统，用于由五个激光组成，并完全包含在BSL2/BSL3 BioSafety橱柜内。我们对该工具的需求的理由是基于三个关键必需品。首先，BD不再为我们的FACSVantage SE提供服务。实际上，自从本申请的原始提交以来，该工具从技术上讲是不合适的。其次，许多理事机构在流式细胞仪中的生物安全水平，遏制和实践的不断发展的策略需要使用带有BSL2/BSL3遏制系统的细胞分类器，其中许多细胞类型都在FCRF处进行细胞分类，并且FACSVANTARGE SE无法完成。第三，对于大多数希望在其研究中应用最先进的技术的用户来说，流式细胞仪的技术进步尤其是细胞分类，使FACSVantage SE成为相当无效且效率低下的细胞分选仪。这些艰难的现实目前正在对FCRF为调查人员提供的服务的可用性造成极大的压力，并且在某些情况下开始妨碍他们的生产力和竞争力，以确保其研究的壁外资金。获取这个新的细胞分类器应允许 IUSM的近五十名调查人员多年来进行研究，以在安全且合规的生物内部维护环境中，并受益于促进一流调查并增强尖端科学的最先进技术。