Whole genome screen for novel regulators of tissue homeostasis and regeneration

全基因组筛选组织稳态和再生的新型调节因子

• 批准号: 8442881
• 负责人: Suzanna E Lewis
• 金额: $ 26.31万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-20 至 2014-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8442881
• 关键词: Address; Adult; Algorithms; Animal Feed; Animal Model; Animals; Biological Models; Caenorhabditis elegans; Cell physiology; Cells; Communities; Computer software; Cultured Cells; Data Analyses; Databases; Drosophila genus; Drosophila melanogaster; Ensure; Environment; Genes; Genetic Techniques; Genome; Goals; Grant; Head; Health; Homeostasis; Homologous Gene; Housing; Human; Image; Image Analysis; Immunologic Techniques; Invertebrates; Libraries; Life; Maintenance; Medical; Metadata; Microscope; Microscopy; Mitotic; Modeling; Molecular; Natural regeneration; Online Mendelian Inheritance In Man; Ontology; Organism; Pathway interactions; Pattern; Phenotype; Phylogenetic Analysis; Planarians; Preparation; Process; RNA Interference; Regulation; Relative (related person); Research; Resources; Sequence Alignment; Series; Stem cells; Techniques; Tissues; Work; Wound Healing; adult stem cell; base; design; feeding; frontier; functional genomics; genome annotation; genome database; genome sequencing; genome wide association study; genome-wide; human stem cells; image archival system; image processing; indexing; innovation; mRNA Expression; new technology; novel; public health relevance; regenerative; repository; research study; screening; tissue regeneration; tool

DESCRIPTION (provided by applicant): Whole genome screen for novel regulators of tissue homeostasis and regeneration Schmidtea mediterranea is a model system for addressing human health issues. Planarians are well known for their ability to regenerate complete animals from fragments of their bodies. Following recent demonstrations that S. mediterranea is amenable to modern cell, molecular, and RNAi techniques, it is becoming the model organism of choice for addressing research questions that cannot be easily studied in Drosophila melanogaster or Caenorhabditis elegans, including wound healing of adult tissues, regeneration, somatic stem cells, and tissue homeostasis. In 2007 we annotated the S. mediterranea genome and constructed a publicly available genome database, SmedGD containing the gene models. The objective of this grant is to use the S. mediterranea gene annotations in a high-throughput image-based screen for novel regulators of tissue regeneration and homeostasis. Toward this end we will (1) employ a battery of molecular and immunological techniques, including a genome-wide RNAi screen, and (2) leverage existing image processing, management and annotation tools to construct a cyberinfrastructure that can both support our experiments and distribute our results to the scientific community.

描述（由申请人提供）：整个基因组筛选组织稳态的新型调节剂和再生Schmidtea Mediterranea是解决人类健康问题的模型系统。平面人以其从身体碎片中再生动物再生动物的能力而闻名。在最近的证明中，地中海链球菌与现代细胞，分子和RNAi技术相吻合，它已成为解决研究问题的模型生物体，用于解决研究问题，这些问题在果蝇中无法轻松研究的秀丽隐杆线虫或Caenorhabditis elegans，包括对成人组织的伤口愈合，再生，体育型干细胞，身体干细胞和细胞托管抑制剂。 2007年，我们注释了地中海链球菌基因组，并构建了一个公开可用的基因组数据库，其中包含基因模型。该赠款的目的是在基于图像的高通量图像屏幕中使用链球菌基因注释，以用于组织再生和稳态的新调节剂。为此，（1）将采用一系列分子和免疫学技术，包括全基因组RNAi屏幕，以及（2）利用现有的图像处理，管理和注释工具来构建网络基础结构，既可以支持我们的实验并将我们的结果分配给科学社区。

项目成果

ImagePlane: an automated image analysis pipeline for high-throughput screens using the planarian Schmidtea mediterranea.

ImagePlane：使用涡虫 Schmidtea mediterranea 进行高通量屏幕的自动图像分析管道。

• DOI: 10.1089/cmb.2013.0025
• 发表时间: 2013
• 期刊: Journal of computational biology : a journal of computational molecular cell biology
• 作者: Flygare,Steven;Campbell,Michael;Ross,RobertMars;Moore,Barry;Yandell,Mark
• 通讯作者: Yandell,Mark