PROTEOMICS CORE

蛋白质组学核心

• 批准号: 8360573
• 负责人: Haining Zhu
• 金额: $ 9.56万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360573
• 关键词: Amino Acid Sequence; Centers of Research Excellence; Chemicals; Computer software; Data Analyses; Detection; Diamond; Distant; Dyes; Funding; Gel; Grant; High Pressure Liquid Chromatography; Individual; Internet; Laboratories; Licensing; Mass Spectrum Analysis; Molecular; National Center for Research Resources; Peptide Mapping; Peptide Sequence Determination; Post-Translational Protein Processing; Postdoctoral Fellow; Preparation; Principal Investigator; Pro-Q aerosol foam; Proteins; Proteomics; Research; Research Infrastructure; Research Personnel; Resources; Robotics; Sampling; Sequence Analysis; Services; Site; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Staining method; Stains; Students; System; Training; United States National Institutes of Health; Water; Work; aerobic respiration control protein; base; cost; data acquisition; design; human disease; mass spectrometer; protein profiling

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The proteomics core is designed to provide quantitative and qualitative protein profiling and identification analysis, as well as post-translational modification and sequence analysis through a combination of 2D gel, mass spectrometry and Edman sequencing analysis services. The core is equipped with Bio-Rad IPG and SDS-PAGE apparatus for 2D gel analysis, a Typhoon phosphorimager/scanner system for detection and quantitation of fluorescent protein dyes (Cy-dyes) and stains (SYPRO-Ruby, Pro-Q Diamond) used to analyze 1D and 2D gels, a Ciphergen SELDI-TOF protein profiling system, an ABI QSTAR II Q-TOF Mass Spectrometer with both MALDI and LC/ESI source capabilities, Waters HPLC systems and multiple PCs for data acquisition and analysis including a site license for PDQuest software and a 5 station site license for Ciphergen software. Fully trained technical staff will assist investigators with the full range of proteomics analytical services. The staff will instruct investigators and their laboratory staff (students, post-doctoral fellows and technicians) in sample preparation, 2D gel analysis and quantitation by fluorescent staining methods. Protein fragmentation, mass peptide mapping and protein sequencing by both MS/MS mass spectrometry and chemical sequencing will be performed by Dr. Beach on samples provided by individual investigators. Preparation and analysis of samples with the Ciphergen system will be facilitated by automated robotic work stations. Data analysis will be performed by investigators and their colleagues through multiple distant work stations connected to the Ciphergen system via the web.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 蛋白质组学核心旨在通过结合 2D 凝胶、质谱和 Edman 测序分析服务，提供定量和定性蛋白质分析和鉴定分析，以及翻译后修饰和序列分析。核心配备用于 2D 凝胶分析的 Bio-Rad IPG 和 SDS-PAGE 装置，以及用于检测和定量荧光蛋白染料（Cy-染料）和染色剂（SYPRO-Ruby、Pro-Q Diamond）的 Typhoon 磷成像仪/扫描仪系统用于分析 1D 和 2D 凝胶、Ciphergen SELDI-TOF 蛋白质分析系统、具有 MALDI 和 MALDI 功能的 ABI QSTAR II Q-TOF 质谱仪LC/ESI 源功能、Waters HPLC 系统和多台用于数据采集和分析的 PC，包括 PDQuest 软件的站点许可证和 Ciphergen 软件的 5 站站点许可证。训练有素的技术人员将协助研究人员提供全方位的蛋白质组学分析服务。工作人员将指导研究人员及其实验室工作人员（学生、博士后研究员和技术人员）进行样品制备、二维凝胶分析和荧光染色定量。 Beach 博士将通过 MS/MS 质谱和化学测序对个别研究人员提供的样品进行蛋白质碎片、质谱肽图分析和蛋白质测序。自动化机器人工作站将有助于使用 Ciphergen 系统制备和分析样品。数据分析将由研究人员及其同事通过通过网络连接到 Ciphergen 系统的多个远程工作站进行。