PATHOGENESIS OF NEONATAL SIV INFECTION

新生儿 SIV 感染的发病机制

• 批准号: 8172914
• 负责人: Ronald S. Veazey
• 金额: $ 6.18万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172914
• 关键词: Acute; Adult; Age; Blood; Bromodeoxyuridine; Cell Proliferation; Cell division; Cells; Childhood; Computer Retrieval of Information on Scientific Projects Database; DNA; Detection; Funding; Grant; HIV Infections; Infection; Institution; Intestines; Macaca; Methods; Monitor; Neonatal; Pathogenesis; Research; Research Personnel; Resources; S Phase; SIV; Source; Spleen; Staging; T-Cell Proliferation; T-Lymphocyte; T-Lymphocyte Subsets; Thymidine; Tissue Banks; Tissues; United States National Institutes of Health; analog; lymph nodes; neonate; peripheral blood

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have been examining neonatal macaques and tissues to determine why SIV/HIV progresses faster in neonates compared to adults. To date, information on T cell turnover rates is limited to peripheral blood, and few studies have examined proliferation and T cell turnover in tissues, particularly the intestinal tract, the primary target for acute SIV and HIV infection. To monitor proliferation of cell subsets in various tissues, we administered bromodeoxyuridine (BrdU) 24 hrs prior to tissue collection. Since BrdU is a thymidine analog incorporated only by cells synthesizing DNA, this method allows for detection of cells in the synthesis phase (S-phase) of cell division. For comparison, cells were also examined for Ki-67 expression, which detects cells at all stages of cell division (G1, S, M, and G2). Proliferation rates of T cell subsets were compared in the blood, lymph nodes, spleen, and intestines of pediatric macaques infected with SIVmac251 and age-matched uninfected controls.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们一直在检查新生儿猕猴和组织，以确定为什么新生儿的 SIV/HIV 进展速度比成人更快。迄今为止，有关 T 细胞周转率的信息仅限于外周血，很少有研究检查组织中的增殖和 T 细胞周转率，特别是肠道，而肠道是急性 SIV 和 HIV 感染的主要目标。为了监测不同组织中细胞亚群的增殖，我们在组织采集前 24 小时施用溴脱氧尿苷 (BrdU)。 由于 BrdU 是仅由合成 DNA 的细胞掺入的胸苷类似物，因此该方法可以检测处于细胞分裂的合成期（S 期）的细胞。 为了进行比较，还检查了细胞的 Ki-67 表达，该表达可检测细胞分裂所有阶段（G1、S、M 和 G2）的细胞。 比较了感染 SIVmac251 的小猕猴和年龄匹配的未感染对照的血液、淋巴结、脾脏和肠道中 T 细胞亚群的增殖率。