Keratocyte role in guidance of corneal nerves

角膜细胞在角膜神经引导中的作用

基本信息

批准号：
8014885
负责人：
Sandeep Jain
金额：
$ 24.73万
依托单位：
UNIVERSITY OF ILLINOIS AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-02-01 至 2014-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8014885
关键词：
Accountability Address Antibodies Area Axon Basic Science Cell Line Cellular biology Chicago Collagen Type XVIII Cornea Coupled Cues Data Ephrin Receptor EphB1 Ephrins Eye Fibroblasts Growth Illinois In Vitro Investigation Keratectomy, Photorefractive, Excimer Laser Keratoplasty Knockout Mice Learning Ligands Mediating Medical Mentored Clinical Scientist Development Award (K08)Mentors Modeling Molecular Molecular Biology Mus Myofibroblast Natural regeneration Nerve Nerve Regeneration Neurobiology Neurons Neurosciences Panthera leo Pattern Perinatal Play Principal Investigator Process Proliferating Research Institute Research Project Grants Research Proposals Role Scientist Signal Transduction Small Interfering RNA Staging Structure Structure of trigeminal ganglion Supervision System Testing Training Transplantation Universities Work Wound Healing axonal guidance biomedical scientist in vivo neuronal guidance novel overexpression programs receptor reinnervation research study response role model symposium

项目摘要

DESCRIPTION (provided by applicant): This is an application for the Mentored Clinical Scientist Development Award to train Dr. Sandeep Jain. Its main educational objective is to provide Dr. Jain with the structured program necessary for him to become an independent biomedical scientist in the area of ocular neurobiology. The specific aims of the proposed educational program consist of Dr. Jain's active participation in 1) didactic graduate course work in molecular biology and neuroscience at the University of Illinois at Chicago, 2) weekly ocular cell and molecular biology seminar and conferences at the Lions of Illinois Eye Research Institute (LIERI) and other medical affiliates of the University of Illinois at Chicago, and 3) a rigorous basic science research project under the supervision of a mentoring committee comprising four established scientists and clinicians. The mentoring committee comprises role models who will provide the applicant with a supportive setting conducive to learning, an ethos and commitment to excellence as well as accountability. The long term objective of the research proposal is to help delineate some of the cornea-resident cellular (keratocyte) and molecular (EphB1/ephrinB1) nerve guidance cues that play a role in the proper growth and patterning of axons during nerve regeneration in the cornea. We propose a novel hypothesis that cornea resident keratocytes play a hitherto unrecognized role of guiding axons during regeneration of corneal nerves. The specific aims of the project are: 1) To correlate the pattern and course of EphB1/ephrinB1 expression with keratocyte activation and nerve regeneration, 2) To determine the effect of keratocyte ephrinBI ligand induced forward signaling on corneal nerve regeneration, and 3) To determine the effect of keratocyte EphB1 receptor induced forward signaling on nerve regeneration.

描述（由申请人提供）：这是一份指导临床科学家发展奖的申请，旨在培训 Sandeep Jain 博士。其主要教育目标是为 Jain 博士提供成为眼神经生物学领域独立生物医学科学家所需的结构化课程。所提议的教育计划的具体目标包括 Jain 博士积极参与 1) 伊利诺伊大学芝加哥分校分子生物学和神经科学研究生课程的教学工作，2) 每周在狮子会举办的眼细胞和分子生物学研讨会和会议。伊利诺伊眼科研究所 (LIERI) 和伊利诺伊大学芝加哥分校的其他医学附属机构，以及 3) 在由四位知名科学家和临床医生组成的指导委员会的监督下进行严格的基础科学研究项目。指导委员会由榜样组成，他们将为申请人提供有利于学习的支持环境、追求卓越的精神和承诺以及责任感。该研究计划的长期目标是帮助描绘一些角膜驻留细胞（角膜细胞）和分子（EphB1/ephrinB1）神经引导线索，这些线索在角膜神经再生过程中轴突的正确生长和模式中发挥作用。我们提出了一个新的假设，即角膜驻留的角膜细胞在角膜神经再生过程中发挥着迄今为止未被认识的引导轴突的作用。该项目的具体目标是：1) 将 EphB1/ephrinB1 表达的模式和过程与角膜细胞激活和神经再生联系起来，2) 确定角膜细胞 ephrinBI 配体诱导的前向信号传导对角膜神经再生的影响，以及 3)确定角膜细胞 EphB1 受体诱导的前向信号传导对神经再生的影响。