Molecular requirements for proliferation of fetal and adult liver progenitors

胎儿和成人肝脏祖细胞增殖的分子需求

基本信息

批准号：
8290443
负责人：
Stacey S Huppert
金额：
$ 4.81万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-07-15 至 2012-08-06
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8290443
关键词：
Ablation Adult Affect Alagille Syndrome Animal Model Architecture Basement membrane Biliary Blood Vessels Cause of Death Cell Communication Cell Lineage Cell Maintenance Cell Maturation Cell Proliferation Cells Characteristics Chemical Injury Chemicals Cholestasis Complex DNA-Binding Proteins Data Defect Development Diet Embryo Embryonic Development Endoderm Endoderm Cell Endothelial Cells Endothelium Epithelial Cells Failure Genetic Genetic Recombination Goals Hepatic Hereditary Disease Injury Invertebrates Knowledge Lead Ligands Liver Liver Regeneration Maintenance Molecular Morphogenesis Mouse Strains Mus Mutation Natural regeneration Operative Surgical Procedures Partial Hepatectomy Pathology Pathway interactions Play Population Process Proliferating Proteins Pseudostratified Epithelium Relative (related person)Reporter Role Signal Transduction Site Stem cells Structure Surgical Injuries Syndrome Time Tissues United States Vertebrates bile duct cell motility chronic liver disease developmental disease fetal insight liver cell proliferation migration mouse model notch protein novel oval cell progenitor response stem tool

项目摘要

Both ligand and receptors of the Notch pathway have been identified as the causitive factors in Allagille syndrome, a complex developmental disorder. A distinguishing characteristic of this syndrome is cholestasis brought on by paucity of bile ducts. Notch signaling, in general, is a critical molecular component for lineage commitment decisions that affect cell maturation relative to neighboring cells. Thus, we hypothesize that Notch signaling during hepatic morphogenesis and/or regeneration controls the lineage commitment and/or cell fate decisions of progenitor cells that underlie formation of the hepatic biliary and vascular architecture. Formation of this architecture is vitally important for normal hepatic function. Thus, the overall goal of this proposal is to identify and define the cell lineages that require Notch signaling for formation of the hepatic architecture, both during normal hepatic morphogenesis and during regeneration in the adult. Two specific aims are proposed. In Aim 1 we will identify and trace the lineage of cells that activate Notch1 during development and adult liver regeneration. In Aim 2 we will determine whether the Notch pathway plays a direct or indirect role in the proliferation and morphogenesis of the hepatoblast progenitor cell population using mouse models generated to specifically delete Notch signaling in the endoderm and endothelial cell lineages. Both Aims will be achieved by taking advantage of pre-existing mouse models that enable lineage tracing and the lineage- specific ablation of Notch signaling. These studies are significant in that they will define the key site(s) of Notch activation during both the development of the liver and its regeneration in adult populations. The knowledge we gain may, in time, enhance our ability to treat chronic liver diseases, which are currently the 7th leading cause of death in the United States.

Notch 通路的配体和受体均已被确定为致病因素阿拉吉尔综合征（一种复杂的发育障碍）的影响因素。一个有特色的该综合征的特征是胆管缺乏引起的胆汁淤积。缺口一般来说，信号传导是谱系定型的关键分子组成部分影响细胞相对于邻近细胞成熟的决定。因此，我们假设肝脏形态发生和/或再生过程中的Notch信号传导控制构成形成基础的祖细胞的谱系定型和/或细胞命运决定肝胆管和血管结构。这种架构的形成至关重要对正常肝功能很重要。因此，该提案的总体目标是识别并定义需要 Notch 信号传导以形成肝脏结构，无论是在正常肝脏形态发生期间还是在成人的再生。提出了两个具体目标。在目标 1 中，我们将确定并追踪在发育和成年肝脏过程中激活 Notch1 的细胞谱系再生。在目标 2 中，我们将确定 Notch 通路是否发挥直接作用或对肝母细胞祖细胞增殖和形态发生的间接作用使用小鼠模型生成的群体，专门删除Notch信号内胚层和内皮细胞谱系。这两个目标都将通过采取利用现有小鼠模型的优势，实现谱系追踪和谱系- Notch 信号传导的特异性消融。这些研究意义重大，因为它们将定义Notch激活的关键位点在成人肝脏的发育和再生过程中。这我们获得的知识可能会及时增强我们治疗慢性肝病的能力，目前是美国第七大死因。