Fully Automated Angiogenesis Drug Discovery Assay in Zebrafish

斑马鱼全自动血管生成药物发现测定

基本信息

批准号：
8441500
负责人：
ANTHONY A Ferrante
金额：
$ 50.84万
依托单位：
PHYSICAL SCIENCES, INC
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-01 至 2015-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8441500
关键词：
Affect Algorithms Angiogenesis Promoter Animals Automation Biological Assay Birth Blood Vessels Clinic Clinical Management Collection Common Neoplasm Computer software Data Defect Deformity Development Disease Dose Embryo Embryonic Development Ensure Environment Evaluation FDA approved Fertilization Fishes Fluorescence Microscopy Government Green Fluorescent Proteins Heart Hemangioma Hour Image Imagery Inborn Genetic Diseases Individual Infant Laboratories Larva Lead Letters Libraries Life Location Macular degeneration Malignant Neoplasms Medical Methods Metric Microscope Modeling Mutation Patients Pattern Persons Phase Phenotype Positioning Attribute Preclinical Drug Evaluation Probability Process Research Software Tools Testing Therapeutic Time Vascular Endothelium Wisconsin Zebrafish angiogenesis assay development base computerized data processing drug development drug discovery high throughput screening human disease image processing improved infancy instrumentation interest malformation medical schools mutant new growth novel physical science programs repaired research study small molecule software development tool

项目摘要

DESCRIPTION (provided by applicant): The zebrafish, Danio rerio, has become widely accepted as a model for human disease. Small molecule screens have been performed to identify modifiers of vascular patterning but this process has been performed at relatively low throughput since the tools for fully automated processing are not yet available. We propose to develop a novel, high-throughput screening assay for angiogenesis promoters. The assay would be based on the use of the SideView" microplate to collect clear images of the vascular pattern in animals that express GFP in their vasculature. During Phase I we: 1) developed novel methods to modulate angiogenesis promoters in the plexus; 2) developed software that locates the zebrafish, identifies the region of interest, and moves that region to the center of the field of view; and 3) developed automated, post-acquisition image processing software that distinguishes wild-type zebrafish embryos from xxx mutant embryos with deformities in the plexus. During Phase II the methods, software and instrumentation will be fully integrated and the assay will be validated in a model screen using the Prestwick Library of FDA- approved compounds.

描述（由申请人提供）：斑马鱼Danio Rerio已被广泛接受为人类疾病的模型。已经执行了小分子筛选以识别血管图案的修饰符，但是此过程以相对较低的吞吐量执行，因为尚无全自动加工工具。我们建议为血管生成启动子开发一种新型的高通量筛查测定法。该测定法将基于使用侧视图的“微板材料收集动物中表达GFP的血管模式的清晰图像。将野生型斑马鱼胚胎与XXX突变体胚胎区分开的图像处理软件将在II阶段中具有畸形。