Genome wide siRNA screen for identifying host factors required for ZAP function

全基因组 siRNA 筛选，用于鉴定 ZAP 功能所需的宿主因子

基本信息

批准号：
8434103
负责人：
MARGARET R MACDONALD
金额：
$ 10.36万
依托单位：
ROCKEFELLER UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-03-01 至 2014-06-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8434103
关键词：
Affect Alphavirus Animals Antiviral Agents Arboviruses Area Arthritis Biological Assay Bioterrorism Boxing Categories Cells Cessation of life Commit Complex Development Disease Disease Outbreaks Ebola virus Encephalitis Exanthema Exhibits Family Fever Filoviridae Frankfurt-Marburg Syndrome Virus Future Genes Goals Human Human Genome Immune response Infection Integration Host Factors Interferons Knowledge Laboratories Lead Letters Mediating National Institute of Allergy and Infectious Disease Natural Immunity Outcome Pathway interactions Patients Process Proteins RNA Helicase Reagent Research Resources Retroviridae Sindbis Virus Small Interfering RNA Specificity Techniques Testing Togaviridae Toxic effect Universities Validation Viral Virus Work Zinc Fingers animal morbidity base cell type cofactor combat genome-wide high throughput screening human morbidity inhibitor/antagonist innovation mortality novel strategies novel therapeutic intervention pathogen prevent protein function prototype screening success treatment strategy virology

项目摘要

DESCRIPTION (provided by applicant): Viruses in the Alphavirus genus of the Togaviridae family cause significant human and animal morbidity and mortality. There is currently no specific treatment for diseases caused by these viruses. The zinc-finger antiviral protein (ZAP) is a host protein that demonstrates potent inhibition of viruses in this genus, as well as viruses in the Retroviridae and Filoviridae families. The objectives of this project are to use a genome-wide siRNA screening approach to identify host factors that are required for, or facilitate, ZAP function. We anticipate the results will help provide a comprehensive picture of the factors important for ZAP function. This information will provide the basis for interpretation of the curret information regarding ZAP's function and will stimulate new hypothesis-driven research directions. Ultimately, ideas for new approaches to treatment may be stimulated by this work, which may help prevent the devastating diseases caused by these viruses.

描述（由申请人提供）：Togaviridae家族α病毒属中的病毒引起了重要的人类和动物的发病率和死亡率。目前尚无针对这些病毒引起的疾病的特定治疗方法。锌指抗病毒蛋白（ZAP）是一种宿主蛋白，可显示该属中病毒的有效抑制作用，以及逆转录病毒和filoviridae家族中的病毒。该项目的目标是使用全基因组siRNA筛选方法来识别或促进ZAP功能所需的宿主因素。我们预计结果将有助于全面了解对ZAP功能重要的因素。该信息将为解释有关ZAP功能的库雷信息的解释提供基础，并刺激新的假设驱动的研究方向。最终，这项工作可能会刺激新治疗方法的想法，这可能有助于防止这些病毒引起的毁灭性疾病。