Acquisition of Covaris E220 and Sciclone G3 systems for high throughput sequencin

收购 Covaris E220 和 Sciclone G3 系统以实现高通量测序

基本信息

批准号：
8247630
负责人：
LUCA COMAI
金额：
$ 31.13万
依托单位：
UNIVERSITY OF CALIFORNIA AT DAVIS
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-09-15 至 2013-09-14
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): Acquisition of Covaris E220 and Sciclone G3 systems for high throughput sequencing library construction The advent of massively parallel sequencing technologies (next-generation sequencing) is with- out doubt the most significant advance in biology since the invention of PCR. The availability of ever-increasing sequencing capabilities at rapidly decreasing cost is changing the paradigm of scientific research. Many exciting scientific questions, particularly in the field of human health, can now be addressed through these emerging sequencing technologies. One such problem is the need for facile, routine preparation of multiple high-quality sequencing libraries that fit the criteria of the connected experimental scenarios and scientific questions. These libraries must be cost- effective, as well as free of contamination and processing errors. The interaction between UC Davis Genome Center and faculty from various biomedical departments has created a center of excellence focused in exploiting the power of next generation sequencers like Illumina, Pacific Biosciences, and Roche 454. Through a substantial investment of capital and human resources, the center has served as a technology hub for providing cutting edge tools and enabling excellence in research. There is one area, however, where the center lacks in equipment and therefore capabilities: high throughput library construction. However, currently the lack of a state-of-the-at high- throughput efficient sequencing library construction platform at the center is hindering the progress of researchers. To resolve this shortcoming, we propose the acquisition of the Covaris E220 high performance sonicator, a state-of-the art DNA fragmentation device that enables efficient and customizable production of size-selected DNA fragments. In addition, we propose the acquisition of the Sciclone G3 Liquid Handling Workstation by Caliper that enables the production of all types of next generation sequencing libraries. Together these two state-of-the-art instruments will facilitate the efficient utilization of existing sequencing resources at the center and greatly augment the productivity of the researchers of this proposal.

描述（由申请人提供）：用于高通量测序库构造Covaris E220和Sciclone G3系统的获取量大平行测序技术的出现（下一代测序）是自PCR发明以来生物学的最重要进步。以迅速降低的成本逐渐增强测序能力的可用性正在改变科学研究的范式。现在可以通过这些新兴的测序技术来解决许多令人兴奋的科学问题，特别是在人类健康领域。这样的问题之一是，需要符合符合连接的实验场景和科学问题标准的多个高质量测序库的简便，常规准备。这些库必须具有成本效益，并且没有污染和处理错误。 UC Davis基因组中心与各个生物医学部门的教职员工之间的互动创造了一个卓越中心，旨在利用Illumina，Pacific Biosciences和Roche 454等下一代序列的力量。通过大量的资本和人力资源投资，该中心是为提供尖锐工具的技术枢纽提供卓越的工具和卓越工具和卓越的研究。但是，有一个区域，中心缺乏设备和功能：高吞吐量图书馆的建设。但是，目前，该中心缺乏最先进的高吞吐量有效测序图书馆构建平台正在阻碍研究人员的进步。为了解决这一缺点，我们提出了Covaris E220高性能超声仪的获取，这是一种最先进的DNA碎片设备，可实现有效且可自定义的大小选择的DNA片段。此外，我们提出了卡位尺对Sciclone G3液体处理工作站的获取，从而可以生产所有类型的下一代测序库。这两种最先进的工具将共同有助于在中心有效利用现有的测序资源，并大大提高该提案研究人员的生产力。

项目成果

期刊论文数量（106）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Effects of pairing on color change and central gene expression in lined seahorses.

DOI：
10.1111/gbb.12812
发表时间：
2022-06
期刊：
Genes, brain, and behavior
影响因子：
0
作者：
通讯作者：

A highly contiguous genome assembly for the California quail (Callipepla californica).

DOI：
10.1093/jhered/esad008
发表时间：
2023-06-22
期刊：
The Journal of heredity
影响因子：
0
作者：
Benham PM;Cicero C;Escalona M;Beraut E;Marimuthu MPA;Nguyen O;Nachman MW;Bowie RCK
通讯作者：
Bowie RCK

Reference genome of the Virginia rail, Rallus limicola.

DOI：
10.1093/jhered/esad026
发表时间：
2023-06-22
期刊：
The Journal of heredity
影响因子：
0
作者：
通讯作者：

Quantitative characterization of all single amino acid variants of a viral capsid-based drug delivery vehicle.

DOI：
10.1038/s41467-018-03783-y
发表时间：
2018-04-11
期刊：
Nature communications
影响因子：
16.6
作者：
Hartman EC;Jakobson CM;Favor AH;Lobba MJ;Álvarez-Benedicto E;Francis MB;Tullman-Ercek D
通讯作者：
Tullman-Ercek D

Levels and Distribution of Salmonella in Naturally Contaminated Cashews.

自然污染腰果中沙门氏菌的水平和分布。