Laboratory Testing Core

实验室测试核心

• 批准号: 8150067
• 负责人: Daniel A Lawrence
• 金额: $ 23万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8150067
• 关键词: Anticoagulants; Antigens; Atherosclerosis; Biological Assay; Bleeding time procedure; Blood Platelets; Chemicals; Coagulation Process; Development; Ensure; Fibrin fragment D; Fibrinolysis; Genomics; Goals; Immunoassay; Individual; Inflammatory; Laboratories; Libraries; Lipids; Measures; Mouse Protein; Mouse Strains; Mus; Obesity; Plasma; Plasminogen Activator; Plasminogen Activator Inhibitor 1; Platelet aggregation; Proteins; Research Personnel; Risk; Screening procedure; Technology; Testing; Thrombosis; Time; Venous Thrombosis; Zebrafish; adipokines; cytokine; hypercholesterolemia; novel; programs; small molecule; success

Core B: Laboratory Testing Core The Testing Laboratory Core B has the goal to serve every project in the Program. It will provide an array of assays assessing fibrinolysis, coagulation, anticoagulant activities, and plasma lipid profiles. The Core will also provide assays of inflammatory cytokines, and adipokines as well as with established kits for mouse proteins associated with thrombosis, atherosclerosis and obesity. The specific assays that are available for each of the projects are indicated below. Core B will also coordinate the high through-put screening of small molecules by the Center for Chemical Genomics. Project 1: Core B will perform analysis of plasma PAI-1 levels for both active and inactive PAI-1 and of plasminogen activator antigen and activity levels. In addition, plasma lipid profiles will be routinely determined and immunoassays of various inflammatory cytokines as well as adipokines will be measured in a mutiplex format using the Luminex Technology. High through-put screening of small molecule compound libraries for the development of novel PAI-1 inactivating agents will also be performed through the Center for Chemical Genomics. Project 2: Core B will perform analysis of plasma PAI-1 levels for both active and inactive PAI-1 and of plasminogen activator antigen and activity levels. Murine plasmas will also be analyzed for increased risk for thrombosis by determining the levels of plasma coagulation, and anticoagulant proteins in various strains of mice at baseline, and after venous thrombosis with or without hypercholesterolemia. D-dimer levels will also be measured. Procoagulant microparticles will also be measured as well as immunoassays of various inflammatory cytokines. Project 3: Core B will assist the investigators off Project 3 by assessing platelet function including bleeding times and platelet aggregation in mice. Fibrinolytic parameters will be determined including PAI-1 and plasminogen activator levels. Immunoassays of various inflammatory cytokines will also be measured in a mutiplex format using the Luminex Technology. Project 4: Core B will assist the investigators of Project 4 by developing novel, assays for coagulation/fibrinolysis and platelet function in Zebrafish. These assays will include immunoassays and functional assays such as clotting times as well as factor assays. The overall goals of the Testing Laboratory Core B are to enhance the success of each individual project by providing rigorous, standardized assays and to ensure that the combined Program is stronger than each of its individual parts.

核心 B：实验室测试核心 测试实验室核心 B 的目标是为该计划中的每个项目提供服务。它将提供一系列 评估纤溶、凝血、抗凝活性和血浆脂质谱的测定。核心将 还提供炎症细胞因子和脂肪因子的测定以及针对小鼠的已建立的试剂盒 与血栓形成、动脉粥样硬化和肥胖相关的蛋白质。可用于的具体检测 每个项目如下所示。核心B还将协调小分子的高通量筛选 化学基因组学中心的分子。 项目 1：核心 B 将分析活性和非活性 PAI-1 的血浆 PAI-1 水平以及 纤溶酶原激活剂抗原和活性水平。此外，血浆脂质谱将常规 确定并测量各种炎症细胞因子以及脂肪因子的免疫测定 使用 Luminex 技术的复用格式。小分子化合物的高通量筛选 用于开发新型 PAI-1 灭活剂的图书馆也将通过该中心进行 化学基因组学。 项目 2：核心 B 将分析活性和非活性 PAI-1 的血浆 PAI-1 水平以及 纤溶酶原激活剂抗原和活性水平。还将分析鼠血浆是否增加风险 通过测定不同菌株的血浆凝固和抗凝蛋白水平来确定血栓形成 基线时以及静脉血栓形成后伴或不伴高胆固醇血症的小鼠。 D-二聚体水平也会 被测量。促凝血微粒以及各种免疫测定也将被测量 炎症细胞因子。 项目 3：核心 B 将通过评估血小板功能（包括出血）来协助项目 3 的研究人员 时间和小鼠血小板聚集。将确定纤溶参数，包括 PAI-1 和 纤溶酶原激活剂水平。各种炎症细胞因子的免疫测定也将在 使用 Luminex 技术的多重格式。 项目 4：核心 B 将通过开发新颖的检测方法来协助项目 4 的研究人员 斑马鱼的凝血/纤溶和血小板功能。这些测定将包括免疫测定和 功能测定，例如凝血时间以及因子测定。 测试实验室核心 B 的总体目标是通过以下方式提高每个单独项目的成功： 提供严格、标准化的检测，并确保组合后的程序比每个程序都更强 它的各个部分。