Protein Microarray

蛋白质微阵列

• 批准号: 8378820
• 负责人: PHILIP Louis FELGNER
• 金额: $ 16.92万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8378820
• 关键词: Animals; Antibodies; Antigens; Arboviruses; Arizona; Bartonella henselae; Bioinformatics; Cloning; Coccidioides; Computer Analysis; Coupled; DNA; Dengue; Development; Devices; Diagnostic; Diagnostic tests; Drug Formulations; Early Diagnosis; Emerging Communicable Diseases; Enzyme-Linked Immunosorbent Assay; Genome; Genomics; Genotype; Goals; Health; Human; Immunoassay; Infection; Infectious Agent; Infectious Diseases Research; Institutes; Laboratories; Lateral; Prevention; Printing; Protein Microchips; Proteins; Proteome; Research Personnel; Sampling; Scanning; Serum; Services; Subunit Vaccines; T-Lymphocyte; Technology; Testing; Translating; Universities; Vaccines; animal resource; base; biodefense; cost; design; immunogenicity; improved; protein purification; research clinical testing

The goal of the Protein Micro-Array Core is to provide investigators with a high throughput genome cloning platform from which protein microarrays can be made for discovery of vaccine and serodiagnostic antigens. The technology to make whole proteome microarrays is well established, and to date the Core has fabricated arrays containing 16,000 proteins from 20 different infectious agents. Antigens identified by array can be are purified and translated to other accessible serodiagnostic platforms, such as dot arrays in the bottom of 96 well plates (multiplex ELISA) or for the development of subunit vaccines. The also core offers probing and scanning, protein purification, fabrication of immunostrips /multiplex ELISAs (macroarrays in 96 well plates), and a full statistical analysis through the UCI Institute for Genomice and Bioinformatics. The core aims to expand on these capabilities by developing low cost DNA-baased genotyping and high throughput T cell antigen discovery. To support activities in the PSWRCE, the core will achieve the following aims Aim i: Provide microarray services and deliverables for new projects: a. Coccidioides posadasii (with John Galgiani, University of Arizona; Project 6.2) b. Bartonella henselae (with Jane Koehler, UCSF) Aim 2: Expand on existing platforms. a. Develop high throughput T cell antigen discovery (Coccidioides posadasii; Project 6.2). b. Develop low-cost DNA-based genotyping test (Dengue). Aim 3: Develop multiplex diagnostic tests to Arboviruses (with Carl Hanson, Immunoassay Development Services Core, and Paul Luciw Animal Resources and Laboratory Services Core). a. Immunostrips/Lateral flow devices b. Multiplex ELISA (Macro-arrays in 96-well plates)

蛋白质微阵列核心的目的是为研究人员提供高通量基因组克隆 可以从中制作蛋白质微阵列以发现疫苗和血清诊断抗原的平台。 制造整个蛋白质组微阵列的技术已经建立了良好，迄今为止，核心已经制造了 来自20种不同传染剂的16,000种蛋白质的阵列。数组鉴定的抗原可以是 纯化并翻译成其他可访问的血清诊断平台，例如96的底部的点阵列 井板（多重ELISA）或用于开发亚基疫苗。核心还提供探测和 扫描，蛋白质纯化，免疫缠绕 /多重ELISA的制造（96井中的宏观阵列 板），以及通过UCI基因组和生物信息学研究所进行完整的统计分析。核心 旨在通过开发低成本基于DNA的基因分型和高吞吐量来扩展这些功能 细胞抗原发现。 为了支持PSWRCE中的活动，核心将实现以下目标 AIM I：为新项目提供微阵列服务和可交付成果： 一个。 Coccidioides Posadasii（与亚利桑那大学的John Galgiani一起；项目6.2） b。 Bartonella Henselae（与Jane Koehler，UCSF） 目标2：扩展现有平台。 一个。发展高吞吐量T细胞抗原发现（Coccidioides posadasii; Project 6.2）。 b。开发基于低成本DNA的基因分型测试（登革热）。 AIM 3：开发多重诊断测试至arboviruses（使用Carl Hanson，Immunoasle Development 服务核心以及Paul Luciw动物资源和实验室服务核心）。 一个。免疫条带/横向流动设备 b。多重ELISA（96孔板中的宏观阵列）