Confocal Microscope for Live Embryo Imaging in a Shared Resource Facility

在共享资源设施中进行活体胚胎成像的共焦显微镜

• 批准号: 7794272
• 负责人: Lawrence S Prince
• 金额: $ 46.02万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794272
• 关键词: 3-Dimensional; Arts; Biological Models; Communities; Development; Developmental Biology; Embryo; Embryonic Development; Event; Fetal Development; Funding; Heart; Humidity; Image; Imagery; Incubators; Kidney; Lasers; Life; Longitudinal Studies; Lung; Microscope; Microscopy; Molecular; Morphogenesis; Organ; Oxygen measurement, partial pressure, arterial; Pancreas; Physiological; Primitive foregut structure; Request for Proposals; Research; Research Infrastructure; Research Personnel; Research Project Grants; Resource Sharing; Resources; Sampling; Scanning; Structure of choroid plexus; System; Temperature; Time; Tissue Engineering; Tissues; Training; Universities; cellular imaging; embryo culture; embryo tissue; flexibility; innovation; instrument

DESCRIPTION (provided by applicant): This proposal requests funds for purchasing a Live Embryo/Live Tissue laser scanning confocal microscope. The Zeiss LSM710 live embryo/live tissue confocal instrument will be incorporated into the Cell Imaging Shared Resource (CISR) at Vanderbilt University, where it will allow users from across the Vanderbilt research community to perform long-term studies of 3-D embryogenesis and tissue morphogenesis. The Zeiss LSM710 confocal system detailed here is especially configured for long-term dynamic studies of living embryos and cultured tissues. Specifically, this system has increased sensitivity, allowing investigators to use reduced laser energy and exposure times. The increased sensitivity of this system allows visualization of living samples while minimizing damage from the excitation laser. A sophisticated live embryo/tissue incubator system will maintain exact physiological conditions with stable temperature, humidity, pH, and oxygen tension for long-term studies of embryogenesis and tissue morphogenesis. The specialized features of this confocal imaging system will therefore provide investigators with the ability to study the cellular and molecular events guiding 3-dimensional morphogenesis of multiple organs during development using new, state of the art approaches. The major user group consists of NIH-funded developmental biologists from multiple Vanderbilt Departments. Their specific research projects will use the Live Embryo Zeiss LSM710 confocal microscope to study embryonal and fetal development of multiple tissues and organs, including, foregut, choroid plexus, pancreas, lung, kidney, and heart. The flexibility of this system will allow studies using many different model systems from multiple species. As part of the Vanderbilt CISR, all major users will obtain expert training on the instrument and will have access to the instrument through the established CISR infrastructure. The Vanderbilt CISR manages a wide variety of microscopy resources for over 300 lab groups and receives outstanding institutional support from Vanderbilt University. Even with the extensive resources available in the Vanderbilt CISR, the Zeiss LSM710 fills a large void by allowing long-term studies of 3-dimensional morphogenesis. The inclusion of the Zeiss LSM710 in the Vanderbilt Cell Imaging Shared Resource will therefore provide NIH-funded investigators with a significantly more innovative approach to study important questions in developmental biology and tissue engineering.

描述（由申请人提供）：该提案要求提供资金购买活胚胎/活组织激光扫描共焦显微镜。蔡司 LSM710 活胚胎/活组织共聚焦仪器将纳入范德比尔特大学的细胞成像共享资源 (CISR)，使范德比尔特研究界的用户能够对 3D 胚胎发生和组织进行长期研究形态发生。这里详细介绍的蔡司 LSM710 共焦系统专门针对活体胚胎和培养组织的长期动态研究而配置。具体来说，该系统提高了灵敏度，使研究人员能够使用更少的激光能量和曝光时间。该系统灵敏度的提高可以实现活体样本的可视化，同时最大限度地减少激发激光造成的损害。复杂的活胚胎/组织培养箱系统将保持精确的生理条件，包括稳定的温度、湿度、pH 值和氧张力，用于胚胎发生和组织形态发生的长期研究。因此，该共焦成像系统的特殊功能将为研究人员提供使用新的、最先进的方法研究在发育过程中引导多个器官的3维形态发生的细胞和分子事件的能力。主要用户组由来自范德比尔特多个部门的 NIH 资助的发育生物学家组成。他们的具体研究项目将使用活胚胎蔡司 LSM710 共焦显微镜来研究胚胎和胎儿多个组织和器官的发育，包括前肠、脉络丛、胰腺、肺、肾和心脏。该系统的灵活性将允许使用来自多个物种的许多不同模型系统进行研究。作为范德比尔特 CISR 的一部分，所有主要用户都将获得该仪器的专家培训，并可以通过已建立的 CISR 基础设施访问该仪器。范德比尔特 CISR 为 300 多个实验室小组管理各种显微镜资源，并获得范德比尔特大学的杰出机构支持。即使 Vanderbilt CISR 拥有大量可用资源，Zeiss LSM710 通过允许对 3 维形态发生进行长期研究来填补了巨大的空白。因此，将蔡司 LSM710 纳入范德比尔特细胞成像共享资源将为 NIH 资助的研究人员提供一种更具创新性的方法来研究发育生物学和组织工程中的重要问题。