MRI Techniques to Measure Whole-Kidney Nephron

测量全肾肾单位的 MRI 技术

• 批准号: 8386445
• 负责人: Kevin M Bennett
• 金额: $ 22.09万
• 依托单位: ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-20 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8386445
• 关键词: Animal Model; Animals; Area; Biodistribution; Contrast Media; Detection; Diabetes Mellitus; Endowment; Foundations; Goals; Human; Hypertension; Image; Imaging Techniques; In Vitro; Individual; Injection of therapeutic agent; Kidney; Kidney Diseases; Kidney Glomerulus; Label; Link; Liver; Magnetic Resonance Imaging; Measures; Molecular; Mus; Nephrons; Obesity; Organ; Predisposition; Process; Proteoglycan; Renal function; Structure; Systemic disease; Techniques; Testing; Time; Tissues; Toxic effect; Work; base; blood filter; glomerular basement membrane; in vivo; intravenous injection; iron oxide; kidney cortex; macromolecule; nanoparticle; novel strategies; pressure

DESCRIPTION (provided by applicant): The broad, long-term goal of this work is to measure kidney nephron and glomerular volume endowment in humans, in vivo. Each nephron contains a glomerulus, which functions as a high-pressure filter of blood macromolecules. Deficits in nephrons and glomeruli have been correlated with renal diseases such as diabetes, obesity, and hypertension. There is currently no noninvasive technique to count the total number of functioning renal glomeruli and nephrons in vivo. Such a technique would enable studies of kidney and systemic disease in humans, and would open a new area of animal studies of the susceptibility to renal disease. We propose that contrast-enhanced MRI, using MRI-detectable nanoparticles targeted to the glomerular basement membrane, can be used to accurately count the total number of glomeruli and measure glomerular volume in the whole, intact kidney. This proposal is focused on establishing this technique in mice. We further propose to assess the accuracy of the technique in the presence of systemic deficits in nephron endowment in mice. Once completed, the proposed work will open the possibility of real-time, in vivo glomerular counts and volume in studies of renal and systemic diseases in animals and humans. PUBLIC HEALTH RELEVANCE: The purpose of this project is to develop a molecular MRI technique to count the number of kidney glomeruli and volume ex vivo and in vivo. This work will lay the foundation for the use of MRI-detectable nanoparticle probes to study single nephron structure and function in animal models and humans.

描述（由申请人提供）：这项工作的广泛、长期目标是在体内测量人体的肾单位和肾小球体积禀赋。每个肾单位都包含一个肾小球，其功能是血液大分子的高压过滤器。肾单位和肾小球的缺陷与糖尿病、肥胖和高血压等肾脏疾病相关。目前还没有无创技术来计算体内功能性肾小球和肾单位的总数。这种技术将使人类肾脏和全身疾病的研究成为可能，并将开辟肾脏疾病易感性动物研究的新领域。我们建议，使用针对肾小球基底膜的 MRI 可检测纳米颗粒进行对比增强 MRI，可用于准确计数肾小球总数并测量整个完整肾脏中的肾小球体积。该提案的重点是在小鼠中建立这种技术。我们进一步建议在小鼠肾单位禀赋存在系统性缺陷的情况下评估该技术的准确性。一旦完成，拟议的工作将为动物和人类肾脏和全身疾病的研究提供实时体内肾小球计数和体积的可能性。 公共卫生相关性：该项目的目的是开发一种分子 MRI 技术来计算离体和体内肾小球的数量和体积。这项工作将为使用 MRI 可检测的纳米颗粒探针研究动物模型和人类的单个肾单位结构和功能奠定基础。