Glycomics Laboratory for the Early Detection of Epithelial Ovarian Cancer

早期检测上皮性卵巢癌的糖组学实验室

基本信息

批准号：
8351959
负责人：
KAREN L. ABBOTT
金额：
$ 35.6万
依托单位：
UNIVERSITY OF GEORGIA
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-09-03 至 2013-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8351959
关键词：
Antibodies Antibody Formation Biological Assay Biological Markers Breast Carcinoma COOH-terminal signal transamidase Cancer Patient Carbohydrates Cell Adhesion Cell Survival Cells Clinic Clinical Complex Data Detection Development Diagnosis Diagnostic Disease Early Diagnosis Epithelial ovarian cancer Glycoconjugates Glycoproteins Goals Grant Greater sac of peritoneum Growth Gynecologic Immune Immunohistochemistry Immunology Knowledge Laboratories Lead Letters Link Malignant - descriptor Malignant Epithelial Cell Malignant Neoplasms Malignant neoplasm of ovary Mammalian Oviducts Marker Discovery Mass Spectrum Analysis Measures Membrane Membrane Proteins Microscopic Modification Molecular Ovary Pennsylvania Phase Plasma Polysaccharides Post-Translational Protein Processing Proteins Proteomics Protocols documentation Recurrence Research Research Personnel Role Sampling Screening for Ovarian Cancer Screening procedure Sensitivity and Specificity Serum Site Specialized Center Specificity Stage at Diagnosis Staging Survival Rate Symptoms Technology Thrombospondin 1 Time Tissues Tumor Cell Invasion Tumor Expansion Tumor Tissue United States Universities Validation Woman advanced disease anticancer research assay development base cancer cell catalyst glycoprotein structure glycosylation human tissue improved insight killings migration multidisciplinary neoplastic cell novel periostin pre-clinical response sugar tumor

项目摘要

DESCRIPTION (provided by applicant): Ovarian cancer is a silent killer with few early symptoms and advanced disease present at the time of diagnosis. This cancer is the most lethal of all gynecologic malignancies with over 20,000 new cases diagnosed each year. The 5 year survival rates for ovarian cancer dramatically improve when the disease is diagnosed at an early stage. Therefore, the long term goal of the Glycomics Laboratory for the Early Detection of Epithelial Ovarian Cancer is to apply the most advanced technologies toward the goal of identifying glycoprotein and glycan markers that can detect ovarian cancer early. We are a multidisciplinary team with expertise in proteomics, glycomics, and immunology from investigators at the Penn Ovarian Cancer Research Center at the University of Pennsylvania (UPENN) and the Complex Carbohydrate Research Center (CCRC) at the University of Georgia. The co-investigators at the CCRC specialize in the mass spectrometry identification of glycan and glycoprotein biomarkers from complex human tissue and serum samples. The co- investigators at UPENN specialize in preclinical validations, antibody production, and the development of biomarkers for ovarian cancer. The aims of this application are (1) Assay development with phase II and phase III validations of previously identified glycosylation modifications on identified biomarkers for EOC, (2) Glycomic identification of new EOC glycoprotein biomarkers, (3) Functional studies to understand the role of GPI anchored protein modification in the development and progression of EOC. PUBLIC HEALTH RELEVANCE: The research proposed is focused on applying the most advanced technologies for the identification of proteins that carry unique sugar (glycan) molecules specific for ovarian cancer. The detection of the glycan present on these proteins will provide a sensitive, specific assay for the early detection of epithelial ovarian cancer. Early detection of ovarian cancer will save lives.

描述（由申请人提供）：卵巢癌是一种无声杀手，早期症状很少，诊断时已是晚期疾病。这种癌症是所有妇科恶性肿瘤中最致命的，每年诊断出超过 20,000 例新病例。如果早期诊断出卵巢癌，则其 5 年生存率会显着提高。因此，上皮性卵巢癌早期检测糖组学实验室的长期目标是应用最先进的技术来实现识别可以早期检测卵巢癌的糖蛋白和聚糖标记物的目标。我们是一支多学科团队，由宾夕法尼亚大学宾夕法尼亚卵巢癌研究中心 (UPENN) 和佐治亚大学复杂碳水化合物研究中心 (CCRC) 的研究人员组成，拥有蛋白质组学、糖组学和免疫学方面的专业知识。 CCRC 的共同研究人员专门从事复杂人体组织和血清样本中聚糖和糖蛋白生物标志物的质谱鉴定。 UPENN 的共同研究人员专门从事临床前验证、抗体生产和卵巢癌生物标志物的开发。本申请的目的是 (1) 对先前确定的 EOC 生物标志物的糖基化修饰进行 II 期和 III 期验证的测定开发，(2) 新 EOC 糖蛋白生物标志物的糖组学鉴定，(3) 功能研究以了解GPI 在 EOC 的发生和进展中锚定蛋白质修饰。公共健康相关性：拟议的研究重点是应用最先进的技术来鉴定携带卵巢癌特异性独特糖（聚糖）分子的蛋白质。对这些蛋白质上存在的聚糖的检测将为上皮性卵巢癌的早期检测提供灵敏、特异的检测方法。早期发现卵巢癌将挽救生命。