Analytical & Phenotyping Core

分析型

• 批准号: 8293475
• 负责人: Andre Diedrich
• 金额: $ 24.61万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8293475
• 关键词: Animals; Autonomic nervous system; Baroreflex; Biochemical; Biological; Blood Plasma Volume; Blood Pressure; Cardiovascular system; Catechols; Clinical; Complement; Data; Databases; Development; Disease; Dopamine-beta-monooxygenase; Electrocardiogram; Evaluation; Feeds; Funding; Genetic; Heart; Heart Rate; Human; Individual; Instruction; Kidney; Knowledge; Laboratory mice; Link; Measurement; Methods; Monitor; Mus; Mutation; Nerve; Neurologic; Norepinephrine; Performance; Peripheral; Phenotype; Regulation; Resources; Sampling; Services; Signal Transduction; Techniques; Testing; Translating; awake; blood pressure regulation; computerized data processing; data reduction; human data; novel; tool; trafficking

The purpose of the Analytical and Phenotyping Core is to provide state-of-the-art cardiovascular and biochemical analysis of sympathetic baroreflex function, including measurements of sympathetic nerve traffic, catechols, and norepinephrine clearance and spillover. This resource has been critical to the recognition and characterization by our group of new disorders involving the autonomic nervous system, such as dopamine-beta-hydroxylase deficiency. A variety of techniques have been established in this Core to support individual projects, including the determination of plasma volume (Project land 4), heart rate and blood pressure variability (Project 1,2,3, and 4), and baroreflex function (Project 1 and 4). Especially Project 2 among all the others will utilize biochemical evaluation of sympathetic activity which complements other methods of autonomic evaluation detailed in the Clinical Core. Expertise in analysis of ultradian rhythms of cardiovascular parameters for phenotyping will be utilized in Project 1, 3, and 4. Project 1 and 4 will use our novel signal processing tools for neurological signals. In addition, we have implemented an animal phenotyping facility, adapting the methods developed to evaluate autonomic function in humans, to the mice which will be used mainly in Project 1. This includes, among other things, monitoring intra-arterial blood pressure in awake unrestricted mice, monitoring renal sympathetic nerve activity, baroreflex testing, osmopressor tests, and high fidelity ECG, in intact and sinoaortic- denervated mice. Genetically-modified mice are increasingly important in translating autonomic knowledge, and this phenotyping mouse laboratory will be essential in this regard. In this funding period, the Core will provide similar biochemical analytical and phenotyping services but with focus on human data including enhanced signal processing, data reduction with automatic database feeds, links between existing databases, development of data exploration tools for all projects.

分析和表型核心的目的是提供交感压力反射功能的最先进的心血管和生化分析，包括交感神经流量、儿茶酚和去甲肾上腺素清除率和溢出的测量。该资源对于我们小组识别和表征涉及自主神经系统的新疾病（例如多巴胺-β-羟化酶缺乏症）至关重要。该核心建立了多种技术来支持各个项目，包括血浆容量的测定（项目土地 4）、心率和血压变异性（项目 1、2、3 和 4）以及压力感受反射功能（项目 1、2、3 和 4）。 1 和 4)。 特别是项目 2 将利用交感神经活动的生化评估，这补充了临床核心中详细介绍的其他自主神经评估方法。项目 1、3 和 4 将利用分析心血管参数超电节律进行表型分析的专业知识。 项目 1 和 4 将使用我们新颖的神经信号信号处理工具。此外，我们还实施了一个动物表型分析设施，将评估人类自主功能的方法应用于主要用于项目 1 的小鼠。这包括在不受限制的清醒状态下监测动脉血压。小鼠，监测完整小鼠和去主动脉神经的小鼠的肾交感神经活动、压力反射测试、渗透压测试和高保真心电图。转基因小鼠在自主神经翻译方面变得越来越重要 知识，而这个表型小鼠实验室在这方面将是至关重要的。在此资助期间，核心将提供类似的生化分析和表型服务，但重点关注人类数据，包括增强信号处理、通过自动数据库馈送进行数据缩减、现有数据库之间的链接、为所有项目开发数据探索工具。