Vector Shared Resource

矢量共享资源

基本信息

批准号：
7944613
负责人：
NORIYUKI KASAHARA
金额：
$ 11.59万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-04-02 至 2013-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7944613
关键词：
Adenovirus Vector Animal Experiments Applications Grants Area Cancer Center Cancer Center Support Grant Cell Culture Techniques Cellular biology Characteristics Consultations Custom Development Disease Dominant-Negative Mutation Epitopes Gene Delivery Gene Expression Regulation Gene Transfer Genes Genetic Genitourinary system Hematopoietic Neoplasms In Vitro Individual Jonsson Comprehensive Cancer Center of University of California Los Angeles Laboratories Libraries Malignant - descriptor Malignant Neoplasms Mammalian Cell Methodology Molecular Epidemiology of Cancer Organogenesis Pathogenesis Preparation Production Recording of previous events Reporting Research Research Activity Research Personnel Resource Sharing Resources Services Signal Transduction Source Techniques Technology Therapeutic Thoracic Oncology Transgenes Translational Research Viral Vector Woman Work anticancer research cancer cell cellular engineering cost design and construction experience gene therapy in vivo interest meetings member molecular imaging mutant new therapeutic target oncology programs research study tumor immunology vector

项目摘要

The Vector Shared Resource (VSR). Advances in techniques for efficient cellular engineering using virusbased gene delivery vehicles ("vectors") have made it feasible to utilize gene transfer as a methodology for elucidating functions of specific genes, by examining consequences of their over-expression or inhibition. The Vector Shared Resource is a new Shared Resource, established to meet the increasing demand both for routine construction and production of viral vectors and for assistance in developing new vectors, both for cell culture and in vivo animal experiments. The objective of the Vector Shared Resource is to promote and Facilitate basic and translational research by providing JCCC investigators with access to vector technologies that enable efficient gene transfer to mammalian cells, both in culture and in vivo. Our services include: (1) provision, at minimal cost, of various pre-made retroviral, lentiviral and adenoviral vector stocks expressing standard marker genes to utilize in preliminary experiments, as well as a library of available vectors expressing various mammalian genes and corresponding inhibitory sequences; (2) construction and production of custom viral vectors that contain a specific sequence(s) of interest (including wild type and mutant cDNAs with or without epitope tags, dominant-negative expression constructs, antisense mRNAs, siRNAs, etc.) for individual researchers; and (3) provision of educational and advisory resources for researchers with limited experience in viral vector technologies, but who wish to utilize such technologies for efficient functional expression of genetic sequences of interest in vitro and/or in vivo. As the use of viral vector technology requires specialized expertise and resources often not found in an individual investigator's laboratory, offering easy access to these technologies, consolidated in the form of a Shared Resource, can significantly facilitate and expand the scope of JCCC research activities. Furthermore, our services are more cost-effective than utilizing the limited commercial sources offering such technologies. Further value is added by customized technical support available from accessible and knowledgeable Shared Resource staff who can work closely with investigators to troubleshoot and optimize gene transfer experiments, assist with institutional regulatory compliance documents and grant proposal submissions and who are actively engaged in development and application of new vector technologies. Initiated in 2003, the Vector Shared Resource has been used by members of the Signal Transduction and Therapeutics, Women's Cancer, Hematopoietic Malignancies, Gene Regulation, Cancer Cell Biology, Tumor Immunology, Thoracic Oncology, Genitourinary Oncology, Molecular Epidemiology and Cancer Molecular Imaging Program Areas for consultation, vector construction, small and large scale preparation of vector stocks and additional services. (Please also see Section 6.2.3 on Shared Resources in the History, Description, Essential Characteristics). 25 Cancer Center members representing 10 Cancer Center Program Areas utilized the services of the Vector Shared Resource during the reporting period. This is a new shared resource.

矢量共享资源 (VSR)。使用基于病毒的高效细胞工程技术的进展基因传递载体（“载体”）使得利用基因转移作为一种方法成为可能通过检查特定基因过度表达或抑制的后果来阐明特定基因的功能。矢量共享资源是一种新的共享资源，旨在满足日益增长的需求用于病毒载体的常规构建和生产以及协助开发新载体，两者均用于细胞培养和体内动物实验。矢量共享资源的目标是促进和通过为 JCCC 研究人员提供载体技术来促进基础研究和转化研究能够在培养物和体内将基因有效转移至哺乳动物细胞。我们的服务包括：（1）以最低成本提供各种预制的逆转录病毒、慢病毒和腺病毒载体库存，表达用于初步实验的标准标记基因，以及可用载体库表达各种哺乳动物基因和相应的抑制序列；（二）建设与生产包含特定目的序列（包括野生型和带或不带表位标签的突变体 cDNA、显性失活表达构建体、反义 mRNA、 siRNA等）供个别研究人员使用； (3) 提供教育和咨询资源研究人员在病毒载体技术方面经验有限，但希望利用这些技术感兴趣的基因序列在体外和/或体内的有效功能表达。由于利用病毒载体技术需要专门的专业知识和资源，而单个研究人员往往无法找到这些知识和资源。实验室，提供对这些技术的轻松访问，并以共享资源的形式进行整合，可以极大地促进和扩大了 JCCC 研究活动的范围。此外，我们的服务是比利用提供此类技术的有限商业资源更具成本效益。进一步的价值是添加了由平易近人、知识渊博的共享资源员工提供的定制技术支持他们可以与研究人员密切合作，解决和优化基因转移实验，协助机构监管合规文件和赠款提案提交以及谁正在积极从事载体新技术的开发与应用。 Vector Shared 发起于 2003 年资源已被信号转导和治疗、女性癌症、造血系统恶性肿瘤、基因调控、癌细胞生物学、肿瘤免疫学、胸部肿瘤学、泌尿生殖肿瘤学、分子流行病学和癌症分子成像项目领域用于咨询、载体构建、载体库存的小规模和大规模制备以及其他服务。（另请参阅第 6.2.3 节“历史记录、描述、基本信息”中的共享资源特征）。代表 10 个癌症中心计划领域的 25 名癌症中心成员使用了报告期内矢量共享资源。这是一个新的共享资源。