COBRE: U NEL: CORE B: MICROSCOPY & BIOIMAGING

COBRE：U NEL：核心 B：显微镜

• 批准号: 7960357
• 负责人: Javier Seravalli
• 金额: $ 8.12万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960357
• 关键词: Arts; Biology; Centers of Research Excellence; Circular Dichroism Spectroscopy; Collaborations; Complement; Computer Retrieval of Information on Scientific Projects Database; Electron Spin Resonance Spectroscopy; Fluorescence; Funding; Grant; Human Resources; Institution; Methods; Microscopy; Mission; Molecular Conformation; Nebraska; Oxidation-Reduction; Property; Proteins; Reaction; Research; Research Personnel; Research Support; Resources; Services; Source; Students; System; Training; United States National Institutes of Health; Universities; bioimaging; electronic structure; graduate student; inhibitor/antagonist; instrument; instrumentation; interest; macromolecule; reaction rate

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This facility provides service support for the research in this proposal. Much of the important information about redox reactions and interactions between redox centers is obtained by microscopy and bioimaging methods. Electron paramagnetic resonance (EPR) spectroscopy is one of the key instruments used to determine the electronic structure of paramagnetic redox centers. This information complements UV-visible and fluorescence spectra of the macromolecule. Circular dichroism (CD) spectroscopy is used to determine some macroscopic properties of a protein, such as overall conformation between macromolecules and between a macromolecule and a substrate or inhibitor governs the rate of reactions. The primary missions of the Microscopy and Bioimaging Core are: + To provide state-of-the-art microscopy and bioimaging instrumentation services to RBC researchers, non-RBC researchers, and outside investigators; + To help facilitate strong collaborations between RBC researchers and non-RBC researchers within and outside the University of Nebraska system; and + To provide high quality microscopy and bioimaging instrumentation training to graduate students, postdoctoral students, and other interested personnel.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该设施为本提案中的研究提供服务支持。 关于氧化还原反应和氧化还原中心之间相互作用的许多重要信息是通过显微镜和生物成像方法获得的。 电子顺磁共振（EPR）光谱是用于确定顺磁性氧化还原中心电子结构的关键仪器之一。 该信息补充了大分子的紫外可见光谱和荧光光谱。 圆二色性 (CD) 光谱用于确定蛋白质的一些宏观特性，例如大分子之间以及大分子与底物或抑制剂之间控制反应速率的整体构象。 显微镜和生物成像核心的主要任务是： + 为红细胞研究人员、非红细胞研究人员和外部研究人员提供最先进的显微镜和生物成像仪器服务； + 帮助促进内布拉斯加大学系统内外 RBC 研究人员和非 RBC 研究人员之间的强有力合作；和 + 为研究生、博士后和其他感兴趣的人员提供高质量的显微镜和生物成像仪器培训。