A research and drug development tool for the real time in vitro study of single c

用于单细胞实时体外研究的研究和药物开发工具

• 批准号: 8590243
• 负责人: Angelo Gaitas
• 金额: $ 41.08万
• 依托单位: KYTARO, INC.
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-15 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8590243
• 关键词: Address; Adhesions; Affinity; Antibodies; Arteriosclerosis; Autoimmune Diseases; Bacteria; Biological Assay; Blood Platelets; Blood Vessels; Bone Marrow Cells; Cell Adhesion; Cell Membrane Proteins; Cell physiology; Cells; Collaborations; Complex; Coronary Arteriosclerosis; Development; Disease; Elements; Extracellular Matrix Proteins; Funding; Hematopoietic stem cells; Hour; In Vitro; Individual; Legal patent; Leukocytes; Life; Ligands; Malignant Neoplasms; Marketing; Measurement; Measures; Michigan; Microscope; Monitor; Neoplasm Metastasis; Organ; Organism; Pathway interactions; Pharmaceutical Preparations; Pharmacologic Substance; Phase; Physiological Processes; Research; Research Personnel; Resolution; Rheumatoid Arthritis; Sampling; Scientist; Signal Pathway; Small Business Innovation Research Grant; Suction; System; Technology; Testing; Time; Tissues; Tumor Promotion; Universities; base; cancer therapy; cantilever; chemokine; drug development; instrument; particle; public health relevance; receptor; research and development; sensor; tool

DESCRIPTION: In this SBIR Phase II PicoCal will develop a high throughput apparatus to measure changes in cell adhesion at the level of individual receptor-ligand interactions in real time. This tool finds critical applications in research and drug development and would allow researchers to study and manipulate adhesion by bioactive compounds to promote or inhibit physiological processes. There is a significant industrial and scientific need to detect changes that can be induced by chemokines or other highly bioactive compounds in order to elucidate the different pathways of signaling mechanisms within a cell and to screen for new key compounds interfering with such pathways. These findings may help to develop new therapies for cancer, arteriosclerosis, and autoimmune diseases, like rheumatoid arthritis. In research and drug development scientists have to study thousands of compounds to determine, if a compound activates or inhibits adhesion. In addition, many diseases such as cancer are complex and require testing multiple analytes and new key compounds for accurate drug development and treatment. There is a need for new tools in drug development to identify bio- active compounds that inhibit or promote adhesion - or modulate subtle changes in receptor affinity. The instrument can also aid researchers in studying the mechanisms of cell adhesion changes such as in tumor promotion and organ-specific metastasis. Key research and pharmaceutical applications include: leukocyte and hematopoietic stem cell adhesion to blood vessels, as well as to other cells (bone marrow niche) or to extracellular matrix (ECM) proteins, platelet adhesion, bacteria and micro-organism adhesion (bio-fouling).

描述：在此SBIR II期中，Picocal将开发高吞吐剂，以实时测量单个受体配体相互作用水平的细胞粘附变化。该工具在研究和药物开发中找到了关键的应用，将使研究人员能够通过生物活性化合物来研究和操纵粘附以促进或抑制生理过程。为了检测趋化因子或其他高度生物活性化合物可以引起的变化，有很大的工业和科学需求，以阐明细胞内信号传导机制的不同途径，并筛选出干扰这种途径的新密钥化合物。这些发现可能有助于开发用于癌症，动脉硬化和自身免疫性疾病（如类风湿关节炎）的新疗法。在研究和药物开发中，科学家必须研究数千种化合物，以确定化合物是否激活或抑制粘附。此外，许多癌症等疾病都是复杂的，需要测试多种分析物和新的关键化合物，以进行准确的药物开发和治疗。在药物开发中需要新的工具来鉴定抑制或促进粘附的生物活性化合物，或调节受体亲和力的微妙变化。该仪器还可以帮助研究人员研究细胞粘附变化的机制，例如肿瘤促进和器官特异性转移。主要的研究和药物应用包括：白细胞和造血干细胞粘附于血管以及其他细胞（骨髓小裂）或细胞外基质（ECM）蛋白，血小板粘附，细菌和微生物粘附（Bio-Fiolion）（Bio-Fouling）。