MULTIFREQUENCY ESR STUDIES OF TAR DNA DYNAMICS

TAR DNA 动力学的多频 ESR 研究

• 批准号: 8364089
• 负责人: Charles P. Scholes
• 金额: $ 0.28万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364089
• 关键词: DNA; Funding; Future; Grant; HIV-1; National Center for Research Resources; Nucleocapsid Proteins; Play; Principal Investigator; Protein Binding; RNA; RNA Probes; Research; Research Infrastructure; Resources; Response Elements; Role; Source; Spin Labels; Structure; Technology; Transactivation; United States National Institutes of Health; Virus Replication; cost; stem

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The HIV-1 Nucleocapsid protein 7 (NCp7) is a small protein that binds to the stem-loop structures of both DNA and RNA. The recognition and folding of NCp7 and DNA/RNA stem loops play an important role in virus replication.The high field ESR was applied in addition to the X-band ESR to investigate the dynamics of Transactivation Response Element (TAR) DNA stem loop and its interaction with NCp7. The nixroxide spin labels were attached to the stem, loop, and bulge regions of TAR DNA, as well as to the 5' and 3' terminals of the DNA. Future studies to compare the multifrequency spectra of complementary TAR DNA and TAR RNA probed by spin labels in the stem, loop, and bulge regions were planned to tell the difference between the dynamics of complementary TAR DNA and TAR RNA.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 HIV-1 Nucleocapsid蛋白7（NCP7）是一种与DNA和RNA的茎环结构结合的小蛋白。 NCP7和DNA/RNA茎环的识别和折叠在病毒复制中起重要作用。除X波段ESR外，还应用了高场ESR来研究反式激活响应元件（TAR）DNA茎环的动力学及其与NCP7的相互作用。尼克罗氧化物自旋标记附着在焦油DNA的茎，环和凸起区域，以及DNA的5'和3'末端。 未来的研究比较了计划在茎，环和凸起区域中自旋标记探测的互补TAR DNA和TAR RNA的多频谱，以说明互补TAR DNA和TAR RNA的动力学之间的差异。