MULTIFREQUENCY ESR STUDIES OF TAR DNA DYNAMICS

TAR DNA 动力学的多频 ESR 研究

• 批准号: 8364089
• 负责人: Charles P. Scholes
• 金额: $ 0.28万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364089
• 关键词: DNA; Funding; Future; Grant; HIV-1; National Center for Research Resources; Nucleocapsid Proteins; Play; Principal Investigator; Protein Binding; RNA; RNA Probes; Research; Research Infrastructure; Resources; Response Elements; Role; Source; Spin Labels; Structure; Technology; Transactivation; United States National Institutes of Health; Virus Replication; cost; stem

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The HIV-1 Nucleocapsid protein 7 (NCp7) is a small protein that binds to the stem-loop structures of both DNA and RNA. The recognition and folding of NCp7 and DNA/RNA stem loops play an important role in virus replication.The high field ESR was applied in addition to the X-band ESR to investigate the dynamics of Transactivation Response Element (TAR) DNA stem loop and its interaction with NCp7. The nixroxide spin labels were attached to the stem, loop, and bulge regions of TAR DNA, as well as to the 5' and 3' terminals of the DNA. Future studies to compare the multifrequency spectra of complementary TAR DNA and TAR RNA probed by spin labels in the stem, loop, and bulge regions were planned to tell the difference between the dynamics of complementary TAR DNA and TAR RNA.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 HIV-1 核衣壳蛋白 7 (NCp7) 是一种小蛋白，可与 DNA 和 RNA 的茎环结构结合。 NCp7和DNA/RNA茎环的识别和折叠在病毒复制中发挥着重要作用。除了X带ESR之外，还应用高场ESR来研究反式激活响应元件（TAR）DNA茎环及其动力学与NCp7的相互作用。氮氧自由基旋转标记附着在 TAR DNA 的茎、环和凸出区域，以及 DNA 的 5' 和 3' 末端。 未来的研究计划通过茎、环和凸出区域的自旋标记来比较互补 TAR DNA 和 TAR RNA 的多频谱，以区分互补 TAR DNA 和 TAR RNA 动力学之间的差异。