DYNAMIC MODES OF SPIN LABELED RNA

自旋标记 RNA 的动态模式

• 批准号: 8364062
• 负责人: KEITH A EARLE
• 金额: $ 0.09万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364062
• 关键词: Algorithms; Biological Models; Complex; Development; Electron Spin Resonance Spectroscopy; Environment; Funding; Grant; HIV; Life Cycle Stages; National Center for Research Resources; Nucleocapsid Proteins; Oligonucleotides; Principal Investigator; Process; Proteins; RNA; RNA-Protein Interaction; Research; Research Infrastructure; Resources; Sodium Chloride; Source; Spin Labels; Structure; Technology; United States National Institutes of Health; Virion; base; cost; flexibility; nanosecond; protein folding; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Protein/RNA interactions in the life cycle of the HIV virion involve complex interactions in a heterogeneous environment. Electron Spin Resonance (ESR) is a powerful experimental tool to study protein/RNA interactions when the interacting species have limited intrinsic structure. Multifrequency ESR is particularly sensitive to details of rotational dynamics on the microsecond to nanosecond timescale, where many biochemically significant processes occur. On the basis of many previous studies and careful numerical algorithm development, it is known how to extract reliable, informative results from multifrequency ESR spectra. We propose to study a model system of spin-labeled TAR-RNA interacting with the protein NCp7 that would be difficult to study by other means due to its flexible structure. In particular, we intend to use multifrequency ESR to study dynamic and structural aspects of hairpin oligonucleotide-nucleocapsid protein folding and recognition. Promising initial results have been obtained at 240 GHz on spin-labeled TAR-RNA with associated NCp7 in a high salt environment.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 在HIV病毒的生命周期中，蛋白质/RNA相互作用涉及在异质环境中的复杂相互作用。当相互作用物种具有有限的内在结构时，电子自旋共振（ESR）是研究蛋白质/RNA相互作用的强大实验工具。多频ESR对微秒至纳米超秒时间尺度上的旋转动力学细节特别敏感，在该时间尺度上，发生了许多具有生物化学意义的过程。在许多先前的研究和仔细的数值算法开发的基础上，已知如何从多频ESR光谱中提取可靠，信息丰富的结果。我们建议研究一种与蛋白质NCP7相互作用的自旋标记的TAR-RNA的模型系统，由于其柔性结构，该模型很难通过其他方式研究。特别是，我们打算使用多频ESR来研究发夹寡核苷酸核苷酸核苷酸蛋白质蛋白折叠和识别的动态和结构方面。 在高盐环境中，在自旋标记的TAR-RNA与相关的NCP7的自旋标记的TAR-RNA上获得了有希望的初始结果。