DYNAMIC MODES OF SPIN LABELED RNA

自旋标记 RNA 的动态模式

• 批准号: 8364062
• 负责人: KEITH A EARLE
• 金额: $ 0.09万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364062
• 关键词: Algorithms; Biological Models; Complex; Development; Electron Spin Resonance Spectroscopy; Environment; Funding; Grant; HIV; Life Cycle Stages; National Center for Research Resources; Nucleocapsid Proteins; Oligonucleotides; Principal Investigator; Process; Proteins; RNA; RNA-Protein Interaction; Research; Research Infrastructure; Resources; Sodium Chloride; Source; Spin Labels; Structure; Technology; United States National Institutes of Health; Virion; base; cost; flexibility; nanosecond; protein folding; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Protein/RNA interactions in the life cycle of the HIV virion involve complex interactions in a heterogeneous environment. Electron Spin Resonance (ESR) is a powerful experimental tool to study protein/RNA interactions when the interacting species have limited intrinsic structure. Multifrequency ESR is particularly sensitive to details of rotational dynamics on the microsecond to nanosecond timescale, where many biochemically significant processes occur. On the basis of many previous studies and careful numerical algorithm development, it is known how to extract reliable, informative results from multifrequency ESR spectra. We propose to study a model system of spin-labeled TAR-RNA interacting with the protein NCp7 that would be difficult to study by other means due to its flexible structure. In particular, we intend to use multifrequency ESR to study dynamic and structural aspects of hairpin oligonucleotide-nucleocapsid protein folding and recognition. Promising initial results have been obtained at 240 GHz on spin-labeled TAR-RNA with associated NCp7 in a high salt environment.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的首席研究员可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 HIV病毒体生命周期中的蛋白质/RNA相互作用涉及异质环境中的复杂相互作用。当相互作用的物种具有有限的内在结构时，电子自旋共振 (ESR) 是研究蛋白质/RNA 相互作用的强大实验工具。多频 ESR 对微秒至纳秒时间尺度上的旋转动力学细节特别敏感，其中发生了许多具有生化意义的过程。在许多先前的研究和仔细的数值算法开发的基础上，我们知道如何从多频 ESR 谱中提取可靠的、信息丰富的结果。我们建议研究自旋标记 TAR-RNA 与蛋白质 NCp7 相互作用的模型系统，由于其灵活的结构，很难通过其他方式进行研究。特别是，我们打算使用多频 ESR 来研究发夹寡核苷酸-核衣壳蛋白折叠和识别的动态和结构方面。 在高盐环境中，自旋标记 TAR-RNA 与相关 NCp7 在 240 GHz 下获得了有希望的初步结果。