SIZE OF NUCLEAR BODIES AND INTERCHROMATIN SPACING IN CHICKEN ERYTHROCYTES

鸡红细胞核体大小和染色质间距

• 批准号: 8362745
• 负责人: ANTHONY EDEN
• 金额: $ 1.96万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362745
• 关键词: A-Form DNA; B-DNA; Cell Nucleus; Cells; Chickens; DNA; Dehydration; Erythrocytes; Funding; Grant; Hydration status; National Center for Research Resources; Nuclear; Nucleosomes; Principal Investigator; Rehydrations; Research; Research Infrastructure; Resources; Source; Testing; United States National Institutes of Health; X ray microscopy; X-Ray Tomography; cost; response; transmission process

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We plan to use transmission X-ray microscopy to investigate morphologic changes in cell nuclei caused by changes in hydration. Do nucleosomes and interchromatin space sizes change in response to dehydration and rehydration because of B-DNA to A-DNA to B-DNA conformational change? We will endeavor to test two hypotheses: 1) The nucleosomes become smaller in response to dehydration and returns to full size upon rehydration because of the B-DNA to A-DNA to B-DNA conformational change. 2) The interchromatin space becomes smaller in response to dehydration and increases during rehydration in response to DNA conformational change.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 我们计划使用透射X射线显微镜研究由水合变化引起的细胞核的形态变化。核小体和染色质空间的大小是否会因B-DNA对A-DNA的脱水和补液而发生变化，从而向B-DNA构象变化变化？我们将努力检验两个假设：1）由于脱水而响应脱水，核小体变小，并且由于B-DNA恢复了全尺寸，因为B-DNA升至A-DNA到B-DNA构象变化。 2）在响应脱水时，染色质空间变小，并且在响应DNA构象变化的补液过程中增加。