STRUCTURE-FUNCTION STUDIES ON PUF60 & ON NPP4

PUF60 的结构功能研究

• 批准号: 8361666
• 负责人: DEMETRIOS BRADDOCK
• 金额: $ 0.18万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361666
• 关键词: Alternative Splicing; Binding; Complex; Data; Development; Disease; Exons; Family; Funding; Genes; Grant; Human; Malignant Neoplasms; Messenger RNA; National Center for Research Resources; Nucleotide pyrophosphatase; Oligonucleotides; Poly U; Principal Investigator; Process; Protein Isoforms; Proteins; RNA Splicing; Reporting; Research; Research Infrastructure; Resolution; Resources; Source; Structure; United States National Institutes of Health; cost; mRNA Precursor; phosphoric diester hydrolase; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Subproject description #1: PUF60 Poly(U)-binding-splicing factor PUF60 is a human protein involved in alternative splicing, a process by which exons of pre-mRNA are reconnected in multiple ways during RNA-splicing. A single gene, thus a single pre-mRNA, can give rise to different mRNAs, resulting in multiple protein isoforms. We collected diffraction data on PUF60 in complex with various oligonucleotides or proteins. Subproject description #2: NPP4 The nucleotide pyrophosphatase/phosphodiesterase (NPP) family is involved in development & disease, including various cancers, yet no structures of the seven human proteins have been reported to date. We collected high-resolution diffraction data for NPP4 & solved the structure. This is the first human NPP to be structurally determined.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 子标记描述＃1：PUF60 聚（u） - 结合切解因子PUF60是一种参与替代剪接的人蛋白，在RNA解析过程中以多​​种方式重新连接了一个替代剪接的过程。 因此，单个基因（因此单个前MRNA）会产生不同的mRNA，从而导致多种蛋白质同工型。 我们在与各种寡核苷酸或蛋白质的复合物中收集了PUF60上的衍射数据。 子标记描述＃2：NPP4 核苷酸焦磷酸酶/磷酸二酯酶（NPP）家族参与了包括各种癌症在内的发育和疾病，但迄今为止尚无七种人类蛋白质的结构。 我们为NPP4收集了高分辨率衍射数据并解决了结构。这是第一个在结构上确定的人类NPP。