STRUCTURE OF THE DIMERIZATION DOMAIN OF DIGEORGE CRITICAL REGION 8

DIGEORGE关键区8的二聚化结构域的结构

• 批准号: 8361692
• 负责人: Feng Guo
• 金额: $ 0.34万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361692
• 关键词: Binding; Complex; Development; Dimerization; Disease; Funding; Grant; Heme; Malignant Neoplasms; Mediating; MicroRNAs; Microprocessor; National Center for Research Resources; Principal Investigator; Process; RNA-Binding Proteins; Reporting; Research; Research Infrastructure; Resources; Source; Structure; Transcript; United States National Institutes of Health; cost; nuclease; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Maturation of microRNAs (miRNAs, approximately 22nt) from long primary transcripts [primary miRNAs (pri-miRNAs)] is regulated during development and is altered in diseases such as cancer. The first processing step is a cleavage mediated by the Microprocessor complex containing the Drosha nuclease and the RNA-binding protein DiGeorge critical region 8 (DGCR8). We previously reported that dimeric DGCR8 binds heme and that the heme-bound DGCR8 is more active than the heme-free form. Here, we identified a conserved dimerization domain in DGCR8.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 在发育过程中调节了长主要转录本（主要miRNA（PRI-MIRNA））的microRNA（miRNA，约22nt）的成熟，并在癌症等疾病中受到改变。第一个处理步骤是由含有Drosha核酸酶和RNA结合蛋白Digeorge临界区域8（DGCR8）的微处理器复合物介导的裂解。我们先前报道了Dimeric DGCR8结合血红素，并且血红素结合的DGCR8比无血红素形式更活跃。在这里，我们在DGCR8中识别了一个保守的二聚化域。