STRUCTURAL STUDIES OF METHYLTRANSFERASE MHAE III BOUND TO SUBSTRATE DNA

甲基转移酶 MHAE III 与底物 DNA 结合的结构研究

• 批准号: 8361671
• 负责人: GREGORY Lawrence VERDINE
• 金额: $ 0.29万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361671
• 关键词: Binding; Catalysis; Chemistry; Cytosine; DNA; Funding; Goals; Grant; Methylation; Methyltransferase; National Center for Research Resources; Principal Investigator; Research; Research Infrastructure; Resources; Source; Structure; United States National Institutes of Health; cost; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. DNA. Though the details of catalysis by DNA cytosine-5 methyltransferases are currently known in great detail, the conformational changes that happen before the chemistry takes place have not yet been described. The mechanism by which DNA cytosine-5 methyltransferases target a particular cytosine for extrusion and later methylation is unknown. Hence our goal is to obtain structures of early intermediates of M.HaeIII bound to DNA before the catalysis occurs.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 脱氧核糖核酸。尽管目前已详细了解DNA胞质-5甲基转移酶的催化细节，但尚未描述化学发生之前发生的构象变化。 DNA胞嘧啶-5甲基转移酶靶向特定的胞嘧啶进行挤出和甲基化的机制尚不清楚。因此，我们的目标是在催化之前获得与DNA结合的早期中间体的结构。