CHARACTERIZATION OF MEMBRANE PROTEIN COMPLEXES

膜蛋白复合物的表征

基本信息

批准号：
8362472
负责人：
RAYMOND C STEVENS
金额：
$ 1.29万
依托单位：
SCRIPPS RESEARCH INSTITUTE, THE
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-05-01 至 2012-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8362472
关键词：
Biological Assay Complex Crystallization Detergents Development Funding Goals Grant Image Individual Lipids Maps Membrane Proteins Microscopy Modeling Molecular Molecular Conformation National Center for Research Resources Principal Investigator Proteins Research Research Infrastructure Resolution Resources Roentgen Rays Source Tertiary Protein Structure Transmission Electron Microscopy United States National Institutes of Health cost protein complex protein protein interaction

Biological Assay Complex Crystallization Detergents Development Funding Goals Grant Image Individual Lipids Maps Membrane Proteins Microscopy Modeling Molecular Molecular Conformation National Center for Research Resources Principal Investigator Proteins Research Research Infrastructure Resolution Resources Roentgen Rays Source Tertiary Protein Structure Transmission Electron Microscopy United States National Institutes of Health cost protein complex protein protein interaction

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项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This project focuses on the development and use of transmission electron microscopy in characterizing membrane protein-protein complexes. We will develop an automated assay to image, select, and classify membrane protein complexes in terms of their aggregation state, size, conformational state and domain arrangement. One goal is to develop a more systematic understanding of the effect of detergents and lipids on the stability and conformation of membrane proteins and their complexes in order to help optimize crystallization efforts. A more ambitious goal is to map out individual protein domains in order to better understand the interactions between proteins in a complex that might be conformationally or structurally heterogeneous. This low resolution information can be combined with high-resolution models of individual subunits or subcomplexes, derived from X-ray and NMR studies, in order to develop a more detailed understanding of the protein- protein interactions within complexes.

该副本是利用资源的众多研究子项目之一由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持而且，副投影的主要研究员可能是其他来源提供的包括其他NIH来源。列出的总费用可能代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。该项目着重于传输电子显微镜的开发和使用表征膜蛋白 - 蛋白质复合物。我们将开发自动化测定图像，选择和对膜蛋白复合物的聚合状态进行分类，大小，构象状态和域排列。一个目标是开发更多系统地了解洗涤剂和脂质对稳定性的影响膜蛋白及其复合物的构象以帮助优化结晶努力。一个更雄心勃勃的目标是在为了更好地了解复合物中蛋白质之间的相互作用构象或结构异质。这个低分辨率的信息可以是与单个亚基或子复合物的高分辨率模型相结合，源自 X射线和NMR研究，以对蛋白质的更详细地理解复合物中的蛋白质相互作用。