CHARACTERIZATION OF MEMBRANE PROTEIN COMPLEXES

膜蛋白复合物的表征

基本信息

批准号：
8169696
负责人：
RAYMOND C STEVENS
金额：
$ 1.29万
依托单位：
SCRIPPS RESEARCH INSTITUTE, THE
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-05-01 至 2011-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8169696
关键词：
Biological Assay Complex Computer Retrieval of Information on Scientific Projects Database Crystallization Detergents Development Funding Goals Grant Image Individual Institution Lipids Maps Membrane Proteins Modeling Molecular Conformation Proteins Research Research Personnel Resolution Resources Roentgen Rays Source Tertiary Protein Structure Transmission Electron Microscopy United States National Institutes of Health protein complex protein protein interaction

Biological Assay Complex Computer Retrieval of Information on Scientific Projects Database Crystallization Detergents Development Funding Goals Grant Image Individual Institution Lipids Maps Membrane Proteins Modeling Molecular Conformation Proteins Research Research Personnel Resolution Resources Roentgen Rays Source Tertiary Protein Structure Transmission Electron Microscopy United States National Institutes of Health protein complex protein protein interaction

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项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project focuses on the development and use of transmission electron microscopy in characterizing membrane protein-protein complexes. We will develop an automated assay to image, select, and classify membrane protein complexes in terms of their aggregation state, size, conformational state and domain arrangement. One goal is to develop a more systematic understanding of the effect of detergents and lipids on the stability and conformation of membrane proteins and their complexes in order to help optimize crystallization efforts. A more ambitious goal is to map out individual protein domains in order to better understand the interactions between proteins in a complex that might be conformationally or structurally heterogeneous. This low resolution information can be combined with high-resolution models of individual subunits or subcomplexes, derived from X-ray and NMR studies, in order to develop a more detailed understanding of the protein-protein interactions within complexes.

该副本是利用众多研究子项目之一由NIH/NCRR资助的中心赠款提供的资源。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是对于中心，这不一定是调查员的机构。该项目的重点是在表征膜蛋白 - 蛋白质复合物中的透射电子显微镜的开发和使用。我们将根据其聚合状态，大小，构象状态和域排列来开发一种自动测定，以形象，选择和分类膜蛋白复合物。一个目标是对洗涤剂和脂质对膜蛋白及其复合物的稳定性和构象的影响有更系统的了解，以帮助优化结晶工作。一个更雄心勃勃的目标是绘制单个蛋白质结构域，以便更好地理解复合物中蛋白质之间的相互作用，这种蛋白质可能在构象上或结构上是异质的。该低分辨率信息可以与源自X射线和NMR研究得出的单个亚基或子复合物的高分辨率模型相结合，以便对复合物中的蛋白质 - 蛋白质相互作用进行更详细的了解。