CHARACTERIZATION OF MEMBRANE PROTEIN COMPLEXES

膜蛋白复合物的表征

• 批准号: 8169696
• 负责人: RAYMOND C STEVENS
• 金额: $ 1.29万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169696
• 关键词: Biological Assay; Complex; Computer Retrieval of Information on Scientific Projects Database; Crystallization; Detergents; Development; Funding; Goals; Grant; Image; Individual; Institution; Lipids; Maps; Membrane Proteins; Modeling; Molecular Conformation; Proteins; Research; Research Personnel; Resolution; Resources; Roentgen Rays; Source; Tertiary Protein Structure; Transmission Electron Microscopy; United States National Institutes of Health; protein complex; protein protein interaction

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project focuses on the development and use of transmission electron microscopy in characterizing membrane protein-protein complexes. We will develop an automated assay to image, select, and classify membrane protein complexes in terms of their aggregation state, size, conformational state and domain arrangement. One goal is to develop a more systematic understanding of the effect of detergents and lipids on the stability and conformation of membrane proteins and their complexes in order to help optimize crystallization efforts. A more ambitious goal is to map out individual protein domains in order to better understand the interactions between proteins in a complex that might be conformationally or structurally heterogeneous. This low resolution information can be combined with high-resolution models of individual subunits or subcomplexes, derived from X-ray and NMR studies, in order to develop a more detailed understanding of the protein-protein interactions within complexes.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该项目的重点是透射电子显微镜的开发和使用，以表征膜蛋白-蛋白质复合物。 我们将开发一种自动化检测方法，根据膜蛋白复合物的聚集状态、大小、构象状态和结构域排列对膜蛋白复合物进行成像、选择和分类。目标之一是更系统地了解去污剂和脂质对膜蛋白及其复合物的稳定性和构象的影响，以帮助优化结晶工作。 一个更雄心勃勃的目标是绘制单个蛋白质结构域，以便更好地了解可能在构象或结构上异质的复合物中蛋白质之间的相互作用。 这种低分辨率信息可以与源自 X 射线和 NMR 研究的单个亚基或子复合物的高分辨率模型相结合，以便更详细地了解复合物内的蛋白质-蛋白质相互作用。