LONGITUDINAL 1H NMR SPECTROSCOPY OF MOUSE HD

小鼠 HD 纵向 1H NMR 波谱

• 批准号: 8362841
• 负责人: JANET M DUBINSKY
• 金额: $ 3.03万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362841
• 关键词: Biochemical; Brain Diseases; Brain region; DNA; Defect; Funding; Genes; Grant; Human; Huntington Disease; Inherited; Magnetic Resonance Imaging; Methods; Molecular; Monitor; Mus; NMR Spectroscopy; National Center for Research Resources; Nerve Degeneration; Neurodegenerative Disorders; Principal Investigator; Process; Research; Research Infrastructure; Resources; Source; Transgenic Mice; United States National Institutes of Health; brain cell; cost; in vivo; magnetic field; mouse model; neurochemistry; programs

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The purpose of this project is to investigate the neurochemical changes occurring during the progression of the Huntington's disease (HD) using transgenic mouse models. Huntington's disease is an inherited neurodegenerative disease resulting from genetically programmed degeneration of brain cells in certain brain regions. Genetically manipulated mice, which DNA contain the defected human gene, are used to mimic this human brain disease. In vivo 1H NMR spectroscopy at high magnetic fields is used for a non-invasive quantification of metabolites from selected mouse brain regions. Non-invasive character of this method enables a longitudinal monitoring of biochemical changes underlying the neurodegeneration. The aim of this project is a better understanding of the mechanisms and processes of the neurodegeneration in HD on the molecular level.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 该项目的目的是研究使用转基因小鼠模型在亨廷顿氏病（HD）进展过程中发生的神经化学变化。亨廷顿氏病是一种遗传性神经退行性疾病，是由于某些大脑区域中脑细胞的基因编程变性而引起的。 DNA包含缺陷的人类基因的遗传操纵小鼠用于模仿这种人脑疾病。高磁场处的体内1H NMR光谱法用于对所选小鼠脑区域的代谢物进行非侵入性定量。该方法的非侵入性可以实现对神经退行性基础的生化变化的纵向监测。该项目的目的是更好地理解分子水平上HD中神经退行性的机制和过程。