3HE PULMONARY VENTILATION IMAGING OF MICE EXPOSED TO OZONE

3HE 暴露于臭氧的小鼠的肺通气成像

• 批准号: 8363225
• 负责人: John Nouls
• 金额: $ 1.23万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363225
• 关键词: 3-Dimensional; Air Pollutants; Animal Model; Animals; Breathing; Cardiovascular system; Data; Defect; Distal; Environmental air flow; Fostering; Funding; Gases; Goals; Grant; Health; Heterogeneity; Hospitalization; Hour; Human; Image; Inbred BALB C Mice; Lead; Location; Lower respiratory tract structure; Lung; Magnetic Resonance Imaging; Metric; Microscopy; Mus; National Center for Research Resources; Ozone; Physiological; Principal Investigator; Program Research Project Grants; Pulmonary Ventilation; Research; Research Infrastructure; Resources; Source; Testing; Tissues; United States National Institutes of Health; cost; imaging modality; in vivo; interest; mortality; mouse model; novel; ozone exposure; respiratory; response; software development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Mike Foster is a valued collaborator who has supplied us with all necessary mouse models needed to test novel imaging methods. His long-standing interest is in the effect of ozone on a variety of pulmonary regulatory mechanisms detailed below. He seeks to obtain pilot data for 3He MRI in ozone-exposed mice so that HP gas MRI can be included as a possible core in a program project grant application that he is planning to put together in the next year. This would lead to considerable sustained funding for small-animal HP gas MRI as a supporting core. Ambient ozone exposure it associated with increased hospitalizations, respiratory illness, and increased cardiovascular mortality. Understanding the biologic mechanisms that regulate the patho-physiologic responses to common urban air pollutants is of considerable interest to human health. Both animal models and humans have shown that the lower respiratory tract is vulnerable to inhalation of ozone; however there is little information known in vivo of the 3-dimensional inhomogeneity within ventilation units serving the distal parenchymal tissues, which apparently persists three to thirty hours post-exposure. The goals of this project are to: a) quantify the ventilation change between control and ozone-exposed mice, specifically the volume of ventilation defects 24 hours post-exposure; b) identify the physical location of ventilation defects within the lungs; c) develop a metric of heterogeneity of the distribution of ventilation defects within the lungs. To achieve those goals, we will acquire one three-dimensional 3He image (120 ml 3He) of each of 4 control BALB/c mice, as well as 4 animals exposed to ozone. Software developed at the center will be used to quantify the volume of ventilation defects and visualize the location of the defects, and expanded to provide a metric of heterogeneity.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 迈克·福斯特（Mike Foster）是一位有价值的合作者，他为我们提供了测试新成像方法所需的所有必要的鼠标模型。他的长期兴趣是臭氧对以下详细介绍的各种肺部调节机制的影响。他试图在暴露于臭氧的小鼠中获得3HE MRI的试点数据，以便可以将HP Gas MRI作为计划项目赠款申请中的可能核心，该应用程序计划在明年汇集。这将导致小动物HP气体MRI作为支持核心的大量持续资金。 环境臭氧暴露与住院增加，呼吸道疾病和心血管死亡率增加有关。了解调节对常见城市空气污染物的病态生理反应的生物学机制对人类健康具有很大的关注。动物模型和人类都表明，下呼吸道容易吸入臭氧。然而，几乎没有信息在用于远端实质组织的通风单元内的三维不均匀性的体内信息，显然在暴露后三到三十小时。 该项目的目标是： a）量化对照和暴露于臭氧的小鼠之间的通风变化，特别是暴露后24小时通风缺陷的体积； b）确定肺部通风缺陷的物理位置； c）发展肺部通风缺陷分布的异质性度量。 为了实现这些目标，我们将获得4个对照BALB/C小鼠的三维3HE图像（120 ml 3He），以及暴露于臭氧的4只动物。在中心开发的软件将用于量化通风缺陷的体积并可视化缺陷的位置，并扩展以提供异质性度量。