MELT TRANSITION AND STRUCTURE OF MEIBOMIAN LIPIDS

睑板腺脂质的熔化转变和结构

• 批准号: 8362358
• 负责人: GERALD M FULLER
• 金额: $ 0.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362358
• 关键词: Air; Body Temperature; Cholesterol Esters; Disease; Esters; Eye; Eye diseases; Fatty Acids; Film; Funding; Goals; Grant; Individual; Influentials; Link; Lipid A; Lipids; Mechanics; National Center for Research Resources; Patients; Principal Investigator; Property; Radiation; Research; Research Infrastructure; Resources; Source; Structure; Surface; Temperature; United States National Institutes of Health; Waxes; chemical property; cost; eye dryness; interest; melting; monolayer; physical property; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Meibomian lipids are the major component of the outermost layer of the tear film, the film that protects the surface of the eye. They are composed of a wide variety of lipid species, the majority of which are long chain wax esters, cholesterol esters and fatty acids. Because it forms the interface with air, the physical and chemical properties of the lipid layer are believed to be influential in tear film stability. We are interested in exploring the structure of Meibomian lipids from individuals with stable tear films and patients with dry eye disease (unstable tear films). Studies of Meibomian lipid monolayers have shown the mechanical properties of these lipids to be quite responsive to temperature. We would like to link these results to changes in the crystalline structure from 25¿C through the melting transition, which will indicate the melt temperature of the lipids. In some forms of dry eye disease the composition of these lipids is different than asymptomatic individuals. If the melt temperature changes significantly compared to body temperature, the ability of the lipids to spread and the mechanical properties of the lipid layer may be modified in such a way that the tear film becomes unstable. The goal of this study is to understand the inherent crystalline structure of Meibum from ?normal? and dry eye subjects as a function of temperature using small angle x-ray scattering. We hope that such studies will contribute to an understanding of which properties promote stability in the tear film, how they change with disease, and how they can be restored for individuals suffering from dry eye.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 Meibomian脂质是泪膜最外层的主要组成部分，该膜保护了眼睛的表面。它们由多种脂质物种组成，其中大多数是长链蜡酯，胆固醇酯和脂肪酸。由于它与空气形成界面，因此据信脂质层的物理和化学特性在泪膜稳定性方面具有影响力。我们有兴趣探索来自患有稳定撕裂膜和干眼症患者（不稳定泪液膜）的个体的Meibomian脂质的结构。对Meibomian脂质单层的研究表明，这些脂质的机械性能对温度非常敏感。我们想将这些结果与晶体结构的变化联系起来，从25°C到熔化过渡，这将表明脂质的熔体温度。在某些形式的干眼病中，这些脂质的组成与不对称个体不同。如果与体温相比，熔体温度显着变化，则脂质的扩散能力和脂质层的机械性能的能力可能会以使泪膜变得不稳定的方式进行修饰。这项研究的目的是了解Meibum的继承晶体结构？使用小角度X射线散射，干眼受试者是温度的函数。我们希望这样的研究将有助于理解哪些特性促进催泪膜中的稳定性，它们如何随疾病的变化以及如何为患有干眼症患者恢复它们。