STRUCTURAL ANALYSIS OF BOXA RNA IN COMPLEX WITH HOST ELONGATION FACTORS INVOLVED

BOXA RNA复合物与宿主延伸因子的结构分析

• 批准号: 8362290
• 负责人: JASON STAGNO
• 金额: $ 0.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362290
• 关键词: Bacteriophage lambda; Bacteriophages; Complex; Crystallography; DNA-Directed RNA Polymerase; Elongation Factor; Funding; Gene Expression; Genetic Transcription; Goals; Grant; Modification; National Center for Research Resources; Principal Investigator; Proteins; RNA; Radiation; Research; Research Infrastructure; Resources; Ribosomal Proteins; Site; Source; System; United States National Institutes of Health; antitermination; cost; protein complex; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Transcription antitermination is one of several mechanisms regulating gene expression. Non-processive antitermination, a system best characterized in bacteriophage lambda, involves structural modification of RNA polymerase (RNAP) by a complex of proteins, enabling transcription through downstream factor-dependent termination sites. The antitermination complex consists of phage protein N, N-utilization RNA control sequences (BoxA and BoxB), and at least four host proteins referred to as N-utilization substances (NusA, NusB, NusE, and NusG). Our goal is to use x-ray crystallography to analyze this complex, particularly the specific interactions between BoxA, NusB, and NusE (ribosomal protein S10) as this stable sub-complex is thought to facilitate complex assembly.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 转录抗电子是调节基因表达的几种机制之一。非过程抗释放性是一种最佳特征在噬菌体lambda中的系统，涉及通过蛋白质复合物对RNA聚合酶（RNAP）进行结构修饰，从而通过下游因子依赖性终止位点实现了转录。抗释放复合物由噬菌体N，N-利用RNA控制序列（BoxA和BoxB）以及至少四种称为N-利用物质（NUSA，NUSB，NUSE，NUSE和NUSG）的宿主蛋白。我们的目标是使用X射线晶体学来分析这种复合物，尤其是Boxa，Nusb和Nuse（核糖体蛋白S10）之间的特定相互作用，因为这种稳定的亚复合物被认为可以促进复杂的组装。