STRUCTURAL ANALYSIS OF BOXA RNA IN COMPLEX WITH HOST ELONGATION FACTORS INVOLVED

BOXA RNA复合物与宿主延伸因子的结构分析

• 批准号: 8362290
• 负责人: JASON STAGNO
• 金额: $ 0.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362290
• 关键词: Bacteriophage lambda; Bacteriophages; Complex; Crystallography; DNA-Directed RNA Polymerase; Elongation Factor; Funding; Gene Expression; Genetic Transcription; Goals; Grant; Modification; National Center for Research Resources; Principal Investigator; Proteins; RNA; Radiation; Research; Research Infrastructure; Resources; Ribosomal Proteins; Site; Source; System; United States National Institutes of Health; antitermination; cost; protein complex; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Transcription antitermination is one of several mechanisms regulating gene expression. Non-processive antitermination, a system best characterized in bacteriophage lambda, involves structural modification of RNA polymerase (RNAP) by a complex of proteins, enabling transcription through downstream factor-dependent termination sites. The antitermination complex consists of phage protein N, N-utilization RNA control sequences (BoxA and BoxB), and at least four host proteins referred to as N-utilization substances (NusA, NusB, NusE, and NusG). Our goal is to use x-ray crystallography to analyze this complex, particularly the specific interactions between BoxA, NusB, and NusE (ribosomal protein S10) as this stable sub-complex is thought to facilitate complex assembly.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的首席研究员可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 转录抗终止是调节基因表达的几种机制之一。非进行性抗终止是 lambda 噬菌体中最典型的系统，涉及蛋白质复合物对 RNA 聚合酶 (RNAP) 的结构修饰，从而能够通过下游因子依赖性终止位点进行转录。抗终止复合物由噬菌体蛋白 N、N-利用 RNA 控制序列（BoxA 和 BoxB）和至少四种被称为 N-利用物质的宿主蛋白（NusA、NusB、NusE 和 NusG）组成。我们的目标是使用 X 射线晶体学来分析该复合物，特别是 BoxA、NusB 和 NusE（核糖体蛋白 S10）之间的特定相互作用，因为这种稳定的子复合物被认为有助于复合物的组装。