MEASUREMENT OF THE SPECTRAL SHIFT OF A NEW CLASS OF DNA BINDING DYES

新型 DNA 结合染料光谱位移的测量

• 批准号: 8361776
• 负责人: PAUL J SMITH
• 金额: $ 2.24万
• 依托单位: LOS ALAMOS NAT SECTY-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361776
• 关键词: Binding; Cell Line; Cells; Collaborations; DNA Binding; Dyes; Flow Cytometry; Fluorescence Spectroscopy; Funding; Grant; Image; In Situ; Life; Measurement; National Center for Research Resources; Performance; Principal Investigator; Research; Research Infrastructure; Resources; Sampling; Sorting - Cell Movement; Source; Staining method; Stains; United States National Institutes of Health; Universities; cost; improved; instrument

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In collaboration with Dr. Paul Smith of Cardiff University, we will characterize the emission spectra of a new class of DNA binding dyes that are rapidly cell permeable and excite in the far red. The Cardiff group has characterized these dyes with a combination of conventional flow measurements, fluorescence spectroscopy of bulk samples and live cell spectral imaging. We will use stained cell lines to characterize the performance of the spectral instrument in the NIR region as compared to the other measurements. We will also use the improved PS cytometer to investigate changes in lifetime of these dyes upon DNA binding, potentially enabling separation of bound and unbound flurorophore in situ.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 我们将与加的夫大学的保罗·史密斯（Paul Smith）合作，我们将描述一种新的DNA绑定染料的发射光谱，这些染料迅速渗透，并在遥远的红色中激发。 加的夫组通过常规流动测量，散装样品的荧光光谱和活细胞光谱成像的结合来表征这些染料。 与其他测量值相比，我们将使用染色的细胞系来表征NIR区域中光谱仪器的性能。 我们还将使用改进的PS细胞仪来研究这些染料在DNA结合后的寿命变化，并有可能在原位实现结合和未结合的flurolorophore的分离。