ID OF FACTOR CODE FOR EXPRESSION DOMAINS OF EVOLUTIONARILY FOREBRAIN ENHANCERS

进化前脑增强剂表达域的因子代码ID

• 批准号: 8363840
• 负责人: JOHN L. R. RUBENSTEIN
• 金额: $ 0.82万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363840
• 关键词: Area; Binding; Code; Enhancers; Funding; Gene Expression; Genetic Enhancer Element; Grant; Mass Spectrum Analysis; Mus; National Center for Research Resources; Nuclear; Pattern; Principal Investigator; Prosencephalon; Proteins; Research; Research Infrastructure; Resources; Source; Specific qualifier value; Telencephalon; United States National Institutes of Health; Ventricular; Work; cost; interest; transcription factor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The telencephalon is divided into many functionally distinct areas. These areas are characterized by different combinations of gene expression, cytoarchitecture, and pattern of connections. A major question in the field is how these areas are specified. Recent work has identified evolutionary conserved enhancer elements that regulate gene expression in sharply demarcated regions in the ventricular zone of the E11.5 mouse cortex. The spatial extent of these regions is regulated by multiple transcription factors. Our lab is interested in identifying these transcription factors using mass spectroscopy. Our approach involves applying nuclear lysate from specific forebrain regions to enhancer elements. The proteins that specifically bind the enhancer will be isolated and identified using mass spectroscopy.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 端脑分为许多功能上不同的区域。 这些区域的特征是基因表达，细胞结构和连接模式的不同组合。 该领域的一个主要问题是如何指定这些区域。 最近的工作确定了进化保守的增强子元件，这些元件调节了E11.5小鼠皮质的心室区域中尖锐划界区域的基因表达。 这些区域的空间范围由多个转录因子调节。 我们的实验室有兴趣使用质谱识别这些转录因子。 我们的方法涉及将特定前脑区域的核裂解物应用于增强子元素。 专门结合增强子的蛋白将使用质谱分离并鉴定。